五角枫组织脱分化与芽增殖的初步研究

针对五角枫(Acer mono Maxim)无性繁殖困难的现状,从探讨五角枫组织脱分化以及芽增殖入手,开展了器官再生的初步研究。结果表明,由消毒的五角枫种子在MS培养基上萌发获得无菌苗,通过无菌苗的叶、子叶和根均可脱分化形成愈伤组织,其中叶的愈伤诱导率最高,其最适诱导培养基为:MS+6-BA 0.5 mg/L+2,4-D1.0 mg/L+NAA 0.5 mg/L;该愈伤的最适增殖培养基为:MS+6-BA 1.0 mg/L+2,4-D 0.5 mg/L+NAA 0.5 mg/L+TDZ 1.5 mg/L。叶诱导的愈伤经适当诱导可产生不定芽与根;幼苗的茎尖可用于芽的增殖,其较适的增殖培养基为MS+TDZ 0.04 mg/L,生根培养基为1/2MS+IBA 1.5 mg/L。通过诱导脱分化与再分化,首次获得了五角枫的再生苗,为五角枫的快繁组培技术体系的建立奠定了一定基础。

Dedifferentiation and Shoot Proliferation of Acer mono Maxim

Aiming at the fact that the vegetative propagation of the Acer mono Maxim,is difficult,this study was conducted to deal with the dedifferentiation and shoot proliferation of Acer Mono Maxim,and the organogenesis of the plantlet.Seeds of Acer mono Maxim,were sterilized and cultivated in MS medium. Leaves,cotyledons and roots from seedlings were used for the dedifferentiation.The result showed that leaves had the highest induction rate of callus and the best medium for callus inducing was MS+6-BA 0.5 mg/L+2,4-D 1.0 mg/L+ NAA0.5 mg/L;the optimal proliferation medium for callus was MS+6-BA 1.0 mg/L+ 2,4-D 0.5 mg/L+ NAA 0.5 mg/L+ TDZ 1.5 mg/L.Appropriate medium for shoot proliferation was MS+ TDZ 0.04 mg/L, and that for the tissue culture rooting was 1/2MS+IBA1.5 mg/L.The regenerated plants of Acer mono Maxim were obtained for the first time through the dedifferentiation and redifferentiation process and a foundation for the techniques of propagation of Acer mono Maxim was established.