板栗主栽品种的遗传多样性及其亲缘关系分析

 采用9 个酶系统的15 个同工酶位点, 对89 个板栗品种进行了遗传多样性分析, 并以品种间的遗传距离构建UPGMA 聚类图, 鉴别板栗品种和评价它们之间的遗传关系。结果表明: ( 1) 我国板栗主要产区遗传多样性较高, 如浙江、山东、湖北和江苏省; ( 2) 在供试的89 个板栗品种中, 除5 个品种外, 其它均可用多位点同工酶对其作专一性鉴定; ( 3) 基于品种间等位酶遗传距离的UPGMA 聚类图将山东、湖北、江苏以及河南的大部分板栗品种分别聚在一起, 体现了在遗传构成上同地域的板栗品种具有遗传关系相近的特征。

Analysis of Genetic Diversity and Genetic Relationships of Chinese Chesmut

Eighty nine traditional cultivars of Chinese chestnut were investigated in present study for genetic diversity,cultivar identification and genetic relationships.Fifteen isozyme loci of 9 enzymes were examined using isoelectric focusing in thin layer polyacrylamide gels.The parameters of genetic diversity and genetic distance were calculated by Biosys 1 and phenogram was constructed by UPGMA clustering analysis with NTSYS software.A high genetic diversity was observed in major chestnut production provinces,such as Zhejiang,Shandong,Hubei and Jiangsu.Eighty four out of total 89 cultivars examined can be uniquely identified by 15 multi locus isozymes.The UPGMA phenogram showed that the majority of cultivar of Shandong,Hubei,Jiangsu and Henan province was clustered together.The traditional cultivars of Chinese chestnut were inclined to clustering of provincial groups.