| Characterization of Citrus tristeza virus Isolates by Indicators and Molecular Biology Methods |
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| 作者姓名： | ZHOU Yan ZHOU Chang-yong SONG Zhen LIU Ke-hong YANG Fang-yun |
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| 作者单位： | Citrus Research Institute, Southwest University/Citrus Research Institute, Chinese Academy of Agricultural Sciences, Chongqing 400712,P. R. China |
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| 摘 要： | Citrus tristeza virus （CTV） exists in citrus as a large number of distinct strains differing in biological characters. The control strategies such as mild strains cross protection （MSCP） require a clear understanding of the characterization of CTV. For better understanding of the structure of CTV population and the relationship between molecular and biological characterization, 72 CTV samples collected from five provinces in China were studied, using biological indexing, p25/Hinf I restriction fragment length polymorphism （RFLP）, multiple molecular markers, and bidirectional RT-PCR assay. The mixture of severe stem pitting isolates was found to be dominant in the field. CTV isolates with p25/Hinf Ⅰ RFLP group 3 and p23/BD-PCR group Ⅰ, Ⅲ were the main cause of epidemics, and most CTV isolates were found to be the mixture of T30 and VT genotypes. More accurate identification of strain mixtures in the field and better understanding of the biological traits of the isolates may be achieved by applying the three molecular detection methods simultaneously.
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| 关 键 词： | 柑橘腐根病毒 分离 特性 检测指标 分子生物学方法 |
| 收稿时间： | 2006-08-24 |
| 修稿时间： | 2007-12-16 |
Characterization of Citrus tristeza virus Isolates by Indicators and Molecular Biology Methods |
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| ZHOU Yan ZHOU Chang-yong SONG Zhen LIU Ke-hong YANG Fang-yun.Characterization of Citrus tristeza virus Isolates by Indicators and Molecular Biology Methods[J].Agricultural Sciences in China,2007,6(5):573-579. |
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| Authors: | ZHOU Yan ZHOU Chang-yong SONG Zhen LIU Ke-hong YANG Fang-yun |
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| Institution: | 1. Applied Sciences University of Isparta, Faculty of Agricultural Sciences and Technologies, Department of Plant Protection, 32260, Isparta, Turkey;2. Applied Sciences University of Isparta, Faculty of Agricultural Sciences and Technologies, Department of Agricultural Biotechnology, 32260, Isparta, Turkey;1. Plant Virology Group, ICGEB Biosafety Outstation, Ca’Tron di Roncade (TV), Italy;2. Institute for Sustainable Plant Protection, National Research Council of Italy (IPSP-CNR), Turin (TO), Italy;3. International Centre for Genetic Engineering and Biotechnology (ICGEB), Trieste (TS), Italy;4. Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, USA |
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| Abstract: | Citrus tristeza virus (CTV) exists in citrus as a large number of distinct strains differing in biological characters. The control strategies such as mild strains cross protection (MSCP) require a clear understanding of the characterization of CTV. For better understanding of the structure of CTV population and the relationship between molecular and biological characterization, 72 CTV samples collected from five provinces in China were studied, using biological indexing, p25/Hinf Ⅰ restriction fragment length polymorphism (RFLP), multiple molecular markers, and bidirectional RT-PCR assay. The mixture of severe stem pitting isolates was found to be dominant in the field. CTV isolates with p25/Hinf Ⅰ RFLP group3 and p23/BD-PCR group Ⅰ, Ⅲ were the main cause of epidemics, and most CTV isolates were found to be the mixture of T30 and VT genotypes. More accurate identification of strain mixtures in the field and better understanding of the biological traits of the isolates may be achieved by applying the three molecular detection methods simultaneously. |
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| Keywords: | Citrus tristeza virus (CTV) biological indexing molecular detection |
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