| 应用低能N+介导法将大豆外源基因转入羊柴成熟种子的初步研究 |
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| 引用本文： | 陈玲玲,那日,乌艳红,栾守泉,正月. 应用低能N+介导法将大豆外源基因转入羊柴成熟种子的初步研究[J]. 草地学报, 2011, 19(6): 1036-1041. DOI: 10.11733/j.issn.1007-0435.2011.06.025 |
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| 作者姓名： | 陈玲玲 那日 乌艳红 栾守泉 正月 |
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| 作者单位： | 1. 内蒙古大学物理科学与技术学院/内蒙古大学离子束生物工程自治区重点实验室, 内蒙古呼和浩特010021;2. 赤峰市农牧科学研究院, 内蒙古赤峰024031 |
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| 基金项目： | 国家自然科学基金(50267001);内蒙古自治区自然科学基金重大项目(200711020101);内蒙古教育厅基金项目(NJ05025)资助 |
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| 摘 要： | 为研究低能N+介导大豆(Glycine max)基因遗传转化的影响,以羊柴(Hedysarum mongolicum)成熟种子为材料,根据不同注入剂量下羊柴种子的发芽率,计算出羊柴种子发芽率的差异性并绘制出剂量-存活率曲线,找出最适宜的转化剂量。结果表明:经离子束(25keV,900·2.6·1013 N+·cm-2)注入后的羊柴成熟种子发芽率的差异性和剂量-存活率曲线可以确定900·2.6·1013 N+·cm-2是最适宜的转化剂量。将处理后的羊柴种子浸入含有大豆质粒DNA的SSC导入介质,24h后观察到受离子束直接作用的胚部有外源基因的导入和表达,检测到的GUS基因瞬间表达率为13%,转化体的PCR检测结果初步证实了大豆基因已经整合到羊柴基因组中。
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| 关 键 词： | 离子束 羊柴 成熟种子 遗传转化 |
| 收稿时间： | 2011-03-02 |
Trasfering Soybean Gene into Hedysarum mongolicum Mature Seeds by Low Energy N~+ Implantation |
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| CHEN Ling-ling , NA Ri , WU Yan-hong , LUAN Shou-quan , ZHENG Yue. Trasfering Soybean Gene into Hedysarum mongolicum Mature Seeds by Low Energy N~+ Implantation[J]. Acta Agrestia Sinica, 2011, 19(6): 1036-1041. DOI: 10.11733/j.issn.1007-0435.2011.06.025 |
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| Authors: | CHEN Ling-ling NA Ri WU Yan-hong LUAN Shou-quan ZHENG Yue |
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| Affiliation: | 1. School of Physical Science and Technology/Key Laboratory of Ion Beam Bioengineering, Inner Mongolia University, Hohhot, Inner Mongolia 010021, China;2. Chifeng Academy of Agricultural and Animal Husbandry Sciences, Chifeng, Inner Mongolia 024031, China |
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| Abstract: | Effects of low energy N+ implantation on Hedysarum mongolicum mature seeds are studied.The germination rates and survival rates of Hedysarum mongolicum dry seeds with different N+ implantation dosages are investigated.Results show that the feasible transformation dosages are 900×2.6×1013 N+·cm-2.The blue cells expressing β-glucuronidase are observed at Hedysarum mongolicum callus with 13% transient expression.PCR analysis of transformed plants shows that the soybean gene had been integrated into Hedysarum mongolicum genome. |
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| Keywords: | Ion beam Hedysarum mongolicum Mature seeds Genetic transformation |
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