| 不同浓度的IGF-1对山羊毛囊干细胞体外培养的影响 |
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| 引用本文： | SHI Ming-yan,史明艳,高雪,杨学义,窦忠英. 不同浓度的IGF-1对山羊毛囊干细胞体外培养的影响[J]. 中国畜牧兽医, 2009, 36(5): 127-129 |
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| 作者姓名： | SHI Ming-yan 史明艳 高雪 杨学义 窦忠英 |
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| 作者单位： | (1.洛阳师范学院生命科学系， 洛阳 471022； 2.中国农业科学院北京畜牧兽医研究所， 北京 100193；3.西北农林科技大学 国家干细胞工程技术研究中心陕西分中心， 杨凌 712100) |
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| 基金项目： | 国家自然科学基金，国家高技术研究发展计划(863计划)，教育部重大科技专项项目 |
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| 摘 要： | 采用2.4 U/mL Dispase酶消化和机械切割法分离毛囊隆突部，经胰酶（0.5 mg/mL胰酶+0.2 mg/mL EDTA）消化，从山羊耳部皮肤分离得到毛囊干细胞，并检测了不同浓度的IGF-1对山羊毛囊干细胞体外培养的作用。结果表明，IGF-1对毛囊干细胞体外培养作用很明显，并且最适IGF-1浓度是10 ng/mL。
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| 关 键 词： | 毛囊干细胞 克隆形成率 IGF-1 体外培养 |
Effect of Different Concentrations of IGF-1 on the Culture of Goat Hair Follicle Stem Cells |
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| SHI Ming-yan,,GAO Xue,YANG Xue-yi,DOU Zhong-ying. Effect of Different Concentrations of IGF-1 on the Culture of Goat Hair Follicle Stem Cells[J]. China Animal Husbandry & Veterinary Medicine, 2009, 36(5): 127-129 |
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| Authors: | SHI Ming-yan GAO Xue YANG Xue-yi DOU Zhong-ying |
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| Affiliation: | (1.Life Science Department of Luoyang Normal College, Luoyang 471022, China; 2.Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 3.Shaanxi Branch of National Stem Cell Engineering Center, Northwest A&F University,Yangling 712100, China) |
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| Abstract: | Hair follicles were isolated from goat ear skin by 2.4 U/mL Dispase digestion and the bulges were separated with special fine needle and digested with 0.5 mg/mL trypsin plus 0.2 mg/mL EDTA.The results showed that cells were cultured in different concentrations of IGF-1,IGF-1 had a good effect on the goat hair follicle stem cells in vitro and the 10 ng/mL was best concentration. |
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| Keywords: | IGF-1 |
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