| 斑蝥素诱导草地贪夜蛾（Spodoptera frugiperda）卵巢细胞系Sf9凋亡的临界条件 |
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| 引用本文： | 张来喜,杨宝东,张民照,王进忠,张爱环,张志勇. 斑蝥素诱导草地贪夜蛾（Spodoptera frugiperda）卵巢细胞系Sf9凋亡的临界条件[J]. 北京农学院学报, 2011, 26(2): 14-17 |
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| 作者姓名： | 张来喜 杨宝东 张民照 王进忠 张爱环 张志勇 |
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| 作者单位： | 农业应用新技术北京市重点实验室,北京农学院植物科学技术学院,北京102206 |
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| 基金项目： | 国家自然科学基金项目(30971938); 北京市教委科技创新平台项目(PXM2008_014207_055164); 北京市高校人才强教计划项目(PHR-IHLB2011-35) |
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| 摘 要： | 为进一步利用昆虫细胞研究斑蝥素对鳞翅目昆虫的毒杀机理,采用细胞形态学观察法和DNA凝胶电泳法研究斑蝥素诱导草地贪夜蛾（Spodoptera frugiperda）卵巢细胞系Sf9细胞凋亡的临界条件。结果表明：供试细胞凋亡率与斑蝥素处理剂量、时间呈现密切相关性;在较低剂量处理下,可观察到细胞凋亡。当斑蝥素质量浓度达到6.25μg/mL,处理细胞3 h或3.125μg/mL处理细胞12 h时,斑蝥素处理Sf9导致细胞出现明显畸形、形成凋亡小体等凋亡症状。DNA Ladder条件要求严格,在冰浴条件下,当斑蝥素处理质量浓度达到6.25μg/mL处理细胞24 h时,或者12.5μg/mL处理细胞12 h时,可观察到明显的DNA梯度条带。
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| 关 键 词： | 斑蝥素 草地贪夜蛾 凋亡 临界条件 Sf9 |
Critical Condition of Apoptosis of Spodoptera frugiperda Sf9 Cells Lead by Cantharidin |
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| ZHANG Lai-xi,YANG Bao-dong,ZANG Min-zhao,WANG Jin-zhong,ZANG Ai-huan,ZANG Zhi-yong. Critical Condition of Apoptosis of Spodoptera frugiperda Sf9 Cells Lead by Cantharidin[J]. Journal of Beijing Agricultural College, 2011, 26(2): 14-17 |
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| Authors: | ZHANG Lai-xi YANG Bao-dong ZANG Min-zhao WANG Jin-zhong ZANG Ai-huan ZANG Zhi-yong |
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| Affiliation: | ZHANG Lai-xi,YANG Bao-dong,ZANG Min-zhao,WANG Jin-zhong,ZANG Ai-huan,ZANG Zhi-yong*(Beijing Key Laboratory of New Technology in Agricultural Application,College of Plant Science and Technology,Beijing University of Agriculture,Beijing 102206,China) |
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| Abstract: | To understand the cantharidin toxicity to lepidopterans,the critical conditions of apoptosis of the Spodoptera frugiperda Sf9 cells led by the nature chemicals were studied by means of cell morphological observation and DNA Ladder.Results showed that Sf9 morphology was changed and presented apoptosis features with deformity and apoptotic body formation at the condition of cantharidin 6.25 g/mL for 3 hours or cantharidin 3.125 g/mL for 12 hours.Ice bath was a necessary condition for DNA Ladder.At the conditi... |
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| Keywords: | Cantharidin Spodoptera frugiperda apoptosis critical condition Sf9 |
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