湖南湘西地区外观健康犬携带狂犬病病毒的调查研究

为了解湖南湘西地区外观健康犬携带狂犬病病毒的情况,我们于2005年9月从湘西狂犬病疫点采集38份扑杀的外观健康的犬脑组织,并于2006年1月在湘西农贸市场采集了168份外观健康的犬脑组织。RT-PCR和直接荧光抗体试验(FAT)检测表明共有5份犬脑标本为阳性,并用乳鼠脑内接种的方法分离得到了5株狂犬病病毒毒株,分子流行病学分析表明这5株病毒均为野毒。在这5份阳性标本,疫点有4份,检出率为10.5%,市场1份,检出率为0.59%。我们的调查结果表明疫点的外观健康犬携带狂犬病病毒的检出率与国内的其它报道接近,而从市场采集的标本的检出率却要低很多。

Investigation on rabies virus carried by dogs with healthy appearance in Xiangxi district Hunan province

In order to study the situation of rabies virus carried by domestic dogs with healthy appearance in Xiangxi, 38 brain tissue samples collected from places of epidemic focus in September, 2005 and 168 brain tissue samples from markets in January, 2006 were detected with RT-PCR and direct fluorescent antibody test (FAT). Among them five samples were positive. Then five wild rabies virus strains were isolated by mice inoculation test (MIT). Among those five positive samples, four samples, accounting for 10.5% (4 of 38), were from the places of epidemic focuses, and one sample accounting for 0.59% (1 of 168) were from markets. The rate of occurrence of rabies virus in epidemic focuses was in accord with that of other domestic reports, but it was much lower in samples from markets.