生长素和细胞分裂素对平邑甜茶组培苗PAs和NO含量的影响

 以平邑甜茶继代组培苗为试材，研究了NAA、IBA、ZT和6-BA作用下组培苗的生长、内源多胺（PAs）和一氧化氮（NO）含量的变化。结果表明，在MS培养基中添加0.02 ~ 1.00 mg·L^-1的NAA或IBA后，外植体鲜样质量增加率和蛋白质含量以及多胺含量和一氧化氮含量均明显增加，其中浓度在0.10 ~ 0.50 mg·L^-1 时增加最高；同样浓度下，NAA处理的外植体鲜样质量增加率和腐胺含量高于IBA。在0.08 ~ 4.00 mg·L^-1范围内，随着ZT或6-BA浓度的升高，外植体鲜样质量增加率、可溶性蛋白含量、多胺含量和一氧化氮含量均逐渐升高；同样浓度下，6-BA处理的外植体鲜样质量增率和腐胺含量高于ZT。生长素和细胞分裂素促进组培苗生长与促进内源PAs和NO的生成相伴随，组培苗生长动态与内源多胺和一氧化氮含量的变化趋势一致。

Effects of Auxin and Cytokinin on PAs Content and NO Generation of Malus hupehensis Rehd. in Vitro

The effects of NAA, IBA, ZT and 6-BA on the growth and the changes on endogenous levels of PAs and nitric oxide were investigated by using regeneration system of Malus hupehensis Rehd. in vitro. As a result, the increase rate of fresh weight and the contents of protein, polyamines (PAs) and nitric oxide (NO) increased significantly in MS medium supplemented with 0.02-1.00 mg·L^-1 NAA or IBA, and the increases were the highest at concentrations of 0.10 - 0.50 mg·L^-1. One more thing，the effects of NAA on the increase rate of fresh weight and putrescine content were higher than that of IBA at the same concentration. With the increase concentration of ZT or 6-BA at 0.08 - 4.0 mg·L^-1, the increase rate of fresh weight, protein, PAs and NO contents increased gradually. Moreover, the effects of 6-BA on increase rate of fresh weight and putrescine content is higher than that of ZT at the same concentration. Auxin and cytokinin promoted the growth accompanied with the elevated production of PAs and NO, and the growth dynamics showed the similar trend with the contents of PAs and NO in explants in vitro.