| 双向电泳中4种常用染色方法的灵敏度比较 |
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| 引用本文： | 范云峰,谢虹,梁建生. 双向电泳中4种常用染色方法的灵敏度比较[J]. 扬州大学学报(农业与生命科学版), 2008, 29(4) |
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| 作者姓名： | 范云峰 谢虹 梁建生 |
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| 作者单位： | 扬州大学,生物科学与技术学院,江苏,扬州,225009 |
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| 基金项目： | 国家自然科学基金资助项目(30571120);;教育部博士点基金资助项目(20061117002) |
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| 摘 要： | 采用SDS-聚丙烯酰胺凝胶电泳技术,比较分析双向电泳常用4种染色方法的灵敏度,以筛选适合蛋白质组学研究所使用的染色方法。结果表明：银染GE法的灵敏度在5 ng的水平,对于BSA为0.5 ng;银染Blum法的灵敏度在10 ng的水平,对于BSA为0.1 ng;银染Schevchenko法的灵敏度在50 ng级的水平,对于BSA为5 ng;普通考染法的灵敏度在0.25μg的水平,对于BSA为0.025μg。根据结果比较,提出适合一般蛋白质组研究分析的快速灵敏的蛋白质染色方法。
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| 关 键 词： | 蛋白质 双向电泳 染色方法 灵敏度 |
Comparison of sensitivities of four protein staining methods in common use in two-dimensional electrophoresis |
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| FAN Yun-feng,XIE Hong,LIANG Jian-sheng. Comparison of sensitivities of four protein staining methods in common use in two-dimensional electrophoresis[J]. Journal of Yangzhou University(Agricultural and Life Science Edition), 2008, 29(4) |
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| Authors: | FAN Yun-feng XIE Hong LIANG Jian-sheng |
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| Affiliation: | Coll of Biosci and Biotech;Yangzhou Univ;Yangzhou 225009;China |
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| Abstract: | In order to choose an appropriate visualization method for proteomics research,the sensitivities of four staining methods in common use in two-dimensional electrophoresis(2-DE) were investigated by using of SDS-polyacrylamide gel electrophoresis(SDS-PAGE).The results showed that the sensitivity of GE silver staining method was at the level of 5 ng,Blum silver staining method was at the level of 10 ng,Schevchenko silver staining method was at the level of 50 ng,whereas Coomassie brilliant blue(CBB) staining ... |
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| Keywords: | protein two-dimensional electrophoresis staining method sensitivity |
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