Hierarchical action and inhibition of plant Dicer-like proteins in antiviral defense |
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Authors: | Deleris Angélique Gallego-Bartolome Javier Bao Jinsong Kasschau Kristin D Carrington James C Voinnet Olivier |
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Institution: | Institut de Biologie Moléculaire des Plantes, CNRS Unité Propre de Recherche (UPR) 2357, 12, rue du Général Zimmer, 67084 Strasbourg Cedex, France. |
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Abstract: | The mechanisms underlying induction and suppression of RNA silencing in the ongoing plant-virus arms race are poorly understood. We show here that virus-derived small RNAs produced by Arabidopsis Dicer-like 4 (DCL4) program an effector complex conferring antiviral immunity. Inhibition of DCL4 by a viral-encoded suppressor revealed the subordinate antiviral activity of DCL2. Accordingly, inactivating both DCL2 and DCL4 was necessary and sufficient to restore systemic infection of a suppressor-deficient virus. The effects of DCL2 were overcome by increasing viral dosage in inoculated leaves, but this could not surmount additional, non-cell autonomous effects of DCL4 specifically preventing viral unloading from the vasculature. These findings define a molecular framework for studying antiviral silencing and defense in plants. |
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