木瓜蛋白酶酶解鸡骨渣的工艺条件优化

为了更好的利用鸡骨渣副产物,研究和优化木瓜蛋白酶水解鸡骨渣的工艺条件.用温度、加酶量、时间进行单因素实验,以水解度、残渣量和总氮回收率为评价指标,通过Box-Behnken响应面分析法优化木瓜蛋白酶水解鸡骨渣的工艺条件.结果表明:木瓜蛋白酶在温度65℃,加酶量0.1％,反应时间1.8h,自然pH值和1∶1的料液比条件下酶解鸡骨渣时,残渣量最低,水解度和总氮回收率最高,分别为24.6％,14.68％和71.68％,验证的优化结果与小试基本一致.说明木瓜蛋白酶适合用于酶解和综合利用鸡骨渣.

Optimization of hydrolysis conditions for chicken bone residues with papain protease

The hydrolysis conditions of papain protease for chicken bone residues were optimized to develop the chicken bone residual by-products.The effects of hydrolysis temperature,enzyme concentration,hydrolysis time,pH and material-liquid ratio on the hydrolysis degree,residual content and total nitrogen recovery were explored to optimize the hydrolysis conditions of papain protease for chicken bone residues through Box-Benhnken response surface analysis on the basis of single-factor experiments.The results showed that the optimal hydrolysis temperature,enzyme concentration and hydrolysis time for chicken bone residues were found to be 65 ℃,0.1%,1.8h,natural pH and 1∶ 1,respectively.Under these conditions,the residual content,hydrolysis degree and total nitrogen recovery were 24.6%,14.68% and 71.68%,respectively.In the pilot test these parameters were in correspondence with the trail test.The conclusion showed that papain protease was suitable for hydrolyzing and developing the chicken bone residue.