| 驱蚊草组培快繁的研究 |
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| 引用本文： | 张勇平,孙骏威,李素芳,余志良. 驱蚊草组培快繁的研究[J]. 安徽农学通报, 2009, 15(5): 41-42 |
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| 作者姓名： | 张勇平 孙骏威 李素芳 余志良 |
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| 作者单位： | [1]中国计量学院生命科学学院,浙江杭州310018 [2]古田山国家级自然保护区,浙江开化324300 |
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| 摘 要： | 为了得到最适合的驱蚊草初代培养、分化培养和生根培养的激素配比，以叶柄为初始材料，对比研究了多篇文献报道的培养基配方。结果表明，驱蚊草的最佳初代培养基为MS＋NAA0．2mg／L＋6-BA2．0mg／L，2，4-D在初代培养中起着负作用；最佳分化培养基为Ms＋NAA0．6mg／L＋6-BA0．75mg／L＋GA30．2mg／L，但有待进一步研究添加GA3的效果；而驱蚊草的最佳生根培养基为1／2MS＋IBA0．3mg／L。
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| 关 键 词： | 驱蚊草 初代培养 分化培养 生根培养 |
Tissue Culture and Rapid Propagation in Mozzie Buster |
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| Affiliation: | Zhang Yongping et al. ( 1 College of Life Sciences, China Jiliang University, Hangzhou 310018, Zhejiang, China; 2 Gutianshan national nature reserve, Kaihua, 324300, Zhejiang, China ) |
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| Abstract: | Various medium formula reported by different references were compared to get the most suitable hormones combination for primary culture, differentiation culture and rooting culture of Mozzie buster using petiole as explant material. The results showed that the best medium formula for primary culture was MS ＋ NAA 0. 2 mg/L ＋ 6 - BA 2. 0 rag/L, and 2,4 - D had negative effect on primary culture of Mozzie buster. Although MS ＋ NAA 0. 6 mg/L ＋ 6 - BA 0. 75 mg/L ＋ GA3 0. 2 mg/L was the most suitable medium formula for differentiation culture, it remained to determine the effect of adding GA3 for further study. 1/2MS ＋ IBA 0. 3 mg/L was the most suitable medium formula for rooting of shoots. |
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| Keywords: | Mozzie buster Primary Culture Differentiation Culture Rooting Culture |
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