藜麦DAPB基因丰度及生物信息学分析

二氢砒啶二羧酸还原酶(DAPB)是赖氨酸合成途径中的一个重要酶,在蛋白质和细胞壁的合成中起着重要作用.本研究基于KEGG总数据库分析表明,藜麦中DAPB的基因拷贝数远高于水稻、玉米及大豆等作物.对藜麦基因组数据筛选得到4个DAPB基因成员,均属于碱性蛋白质,氨基酸序列长度约330 bp,分子量为36.623～36.88...

Gene Abundance and Bioinformatics Analysis of the DAPB Gene in Quinoa

Dihydrodipicolinate reductase(DAPB)is an important enzyme in lysine synthesis,which also plays a vital role in protein synthesis and cell wall formation.Based on KEGG database analysis,we found the gene copy of DAPB in quinoa was much higher than that in rice,maize and soybean.To understand the function of DAPB gene,in the present study,four DAPB gene members were identified from the Chenopodium quinoa genome and their bioinformatics was analyzed.All the four DAPB are alkaline proteins,and amino acid sequences are about 330 bp.Molecular weights of DAPB are 36.623 k D to 36.880 k D,the theoretical isoelectric point(p I)ranges from 5.79 to6.01,and the maximum hydrophobic coefficient is between 0.028 and 0.123.The proteins locate in cytoplasm without transmembrane region presented.The Ka/Ks of the four DAPB genes are all less than 0.5,which indicate they were conserved during evolution process.Phylogenetic tree presented that the four DAPB genes were in the same subfamily,of which two of them were closely related to Spinacia oleracea L.DAPB promoter homeo-pathic elements analysis showed that besides TATA-box and CAAT-box,DAPB genes also contained multiple cis-acting elements and transcription factor binding sites that involved in hormone,light,low temperature and other st-ress responses,suggested that quinoa DAPB gene family might get involved in above factors response.The present study compared the gene copy of DAPB and conducted bioinformatics analysis of DAPB,which could provide information for further DAPB function study.