| 线粒体介导的三倍体虹鳟卵母细胞凋亡 |
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| 引用本文： | 韩英,马凯,王彦娜,吕晓楠,董宏伟,张振利,薛淑群.线粒体介导的三倍体虹鳟卵母细胞凋亡[J].东北农业大学学报,2019,50(12):51-57. |
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| 作者姓名： | 韩英 马凯 王彦娜 吕晓楠 董宏伟 张振利 薛淑群 |
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| 作者单位： | 东北农业大学动物科学技术学院,哈尔滨,150030;北京市水产技术推广总站,北京,100176;哈尔滨市农业科学院,哈尔滨,150028;黑龙江省水生动物资源增殖保护站,黑龙江 佳木斯,154002 |
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| 摘 要： | 采集不同发育时期三倍体虹鳟幼鱼卵巢,采用免疫组化、线粒体膜电位荧光探针、荧光定量PCR和蛋白免疫印迹等方法,观察、检测卵巢发育过程中卵母细胞细胞凋亡现象及线粒体凋亡途径,证实三倍体虹鳟卵母细胞发生线粒体介导的细胞凋亡。结果表明,12℃发育水温下,三倍体虹鳟卵母细胞在受精后210 d开始凋亡,受精后270 d凋亡达高峰,330 d凋亡逐渐消失。证明卵母细胞发生凋亡是导致三倍体雌性虹鳟不育的重要原因,为三倍体鱼类性腺发育深入研究提供理论依据。
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| 关 键 词： | 三倍体虹鳟 卵母细胞 线粒体 细胞凋亡 |
Mitochondrial-mediated apoptosis in triploid rainbow trout(Oncorhynchus mykiss) oocytes |
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| Institution: | ,School of Animal Sciences and Technology, Northeast Agricultural University,Beijing Aquatic Product Technology Promotion Department,Harbin Academy of Agricultural Science,The Protection Station of Aquatic Animal Resources in Heilongjiang Province |
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| Abstract: | The ovaries of triploid female rainbow trout were sampled at different developmental stages. The apoptosis of oocytes and the apoptotic pathway of mitochondria were detected by immunohistochemistry, mitochondrial membrane potential fluorescence probe, real-time quantitative PCR and Western blot. It was confirmed that the oocytes of triploid rainbow trout occurred mitochondrialmediated apoptosis. The results showed that at 12 ℃, the oocytes of triploid rainbow trout began to apoptosis at 210 days post fertilization(dpf), peaked at 270 dpf, and gradually disappeared at 330 dpf.The findings showed that the apoptosis of oocytes was an important reason for the sterility of triploid female rainbow trout and provided a theoretical basis for further study of gonadal development in triploid fish. |
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| Keywords: | triploid rainbow trout oocyte mitochondrion apoptosis |
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