甘蓝型油菜热激蛋白HSP70基因家族的鉴定和分析

热休克蛋白也称热激蛋白(heat shock protein,HSP),是植物在受到生长环境中不利的生长因子胁迫时产生的一种用于抗逆的防御蛋白,其中在生物体中分布最普遍的一种热激蛋白是热休克蛋白70(heat shock protein 70,HSP70).为全面、深入地了解芸薹属HSP70基因家族的信息,本研究对芸薹...

Identification and Analysis of the Heat Shock Protein HSP70 Gene Family in Brassica napus

Heat Shock Protein(HSP)is a kind of defense protein for stress resistance produced by plants when they are suffering from adverse growth factors in the growing environment.Heat Shock Protein 70(HSP70)is the most widely distributed Heat Shock Protein in organisms among HSP family.In order to comprehensively and deeply understand the information of Brassica HSP70 gene family,bioinformatics analysis of three species in Brassica(Brassica rapa,Brassica oleracea and Brassica napus)has been conducted in this study.First,the number of HSP70 genes in Brassica was identified in the BRAD database.Two HSP70 genes were identified in Brassica rapa and Brassica oleracea respectively,and four HSP70 genes were identified in Brassica napus.The eight HSP70 genes identified in the previous step were analyzed by bioinformatics(Phylogenetic relationship,gene structure,conserved motifs,chromosomal localization,homology,etc.).The results showed that Brassica HSP70 gene was similar in structure and highly conserved in nucleic acid and protein levels.In the process of evolution,the homology in the basic species and between the natural hybrids and the basic species is very high.Phylogenetic tree and other bioinformatics analysis results also showed that the HSP70 site in Brassica rapa,Brassica oleracea and Brassica napus are consistent with the U’s triangle.However,according to the number of HSP70 and the corresponding relationship in the three species in Brassica,an analysis result can be inferred that the HSP70 site of Brassica rapa,Brassica oleracea and Brassica napus are not completely conform to the triploid theory.This result can reflect that the HSP70 gene of these three species of brassica has changed in number or structure during the evolutionary process,which makes it not consistent with triploid theory.