西瓜FBA基因的鉴定及其对不同逆境的响应模式分析

果糖1,6-二磷酸醛缩酶(FBA)是植物体内的一种参与糖酵解、糖异生和卡尔文循环的关键酶。FBA基因已被证实在植物生长发育及多种生物和非生物胁迫响应过程中发挥重要的作用,但目前对西瓜中FBA基因的研究却几乎空白。本研究采用生物信息学手段在西瓜基因组中共鉴定到5个Cl FBA基因,并对它们的保守结构域、基因结构、染色体位置、进化关系、亚细胞定位、基因复制事件、启动子顺式作用元件等进行预测和分析。根据进化和亚细胞定位分析,西瓜FBA蛋白主要分为两类,分别主要定位在叶绿体和细胞质中。此外,我们还利用荧光定量PCR对Cl FBA基因的时空表达模式进行分析,发现大部分西瓜Cl FBA基因在茎和果实中优势表达。同时对西瓜Cl FBA基因在不同生物和非生物逆境处理后的响应模式进行分析,鉴定到一批不同胁迫响应的Cl FBA基因,为西瓜的抗病和抗逆育种提供了重要的分子基础。

Genome-wide Analysis of Watermelon FBA Genes and Their Expression Patterns in Response to Various Stresses

Fructose-1,6-isphospho alactase(FBA)is a key enzyme in glycolysis process,gluconeogenesis process and Calvin cycle in plants.FBA gene plays an important role in the plant growth and various biotic and abiotic stress responses.However,there are few studies on FBA genes in watermelon.Here,we identified five watermelon FBA genes in genome wide level.And their conserved domains,gene structures,chromosome locations,phylogenetic relationships,subcellular localizations,gene duplication events,cis-elements of promoters were also analyzed.According to the phylogenetic evolution and subcellular localization analyses,these watermelon FBA proteins were divided into two type,mainly located in chloroplast and cytoplasmic,respectively.Additionally,Quantitative Real-time PCR was used to analyze the expression level of the Cl FBA gene in various organs and tissues of watermelon,and results indicated most watermelon Cl FBA genes were predominantly expressed in the stem and fruit.We further analyzed the expression patterns of watermelon FBA genes in response to various biotic and abiotic stresses,and some stress-responsive Cl FBA genes were identified.Our study is conducive to the watermelon disease resistance and resistance breeding.