| 谷子丙酮酸磷酸双激酶基因(SiPPDK1)对非生物逆境的响应 |
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| 引用本文： | 杜艳伟,王高鸿,李颜方,赵根有,阎晓光,王振华,王玉文,余爱丽,赵晋锋. 谷子丙酮酸磷酸双激酶基因(SiPPDK1)对非生物逆境的响应[J]. 分子植物育种, 2021, 19(1): 13-21 |
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| 作者姓名： | 杜艳伟 王高鸿 李颜方 赵根有 阎晓光 王振华 王玉文 余爱丽 赵晋锋 |
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| 作者单位： | 山西省农业科学院谷子研究所,特色杂粮种质资源发掘与育种山西省重点实验室,长治,046011;山西省农业科学院谷子研究所,特色杂粮种质资源发掘与育种山西省重点实验室,长治,046011;山西省农业科学院谷子研究所,特色杂粮种质资源发掘与育种山西省重点实验室,长治,046011;山西省农业科学院谷子研究所,特色杂粮种质资源发掘与育种山西省重点实验室,长治,046011;山西省农业科学院谷子研究所,特色杂粮种质资源发掘与育种山西省重点实验室,长治,046011;山西省农业科学院谷子研究所,特色杂粮种质资源发掘与育种山西省重点实验室,长治,046011;山西省农业科学院谷子研究所,特色杂粮种质资源发掘与育种山西省重点实验室,长治,046011;山西省农业科学院谷子研究所,特色杂粮种质资源发掘与育种山西省重点实验室,长治,046011;山西省农业科学院谷子研究所,特色杂粮种质资源发掘与育种山西省重点实验室,长治,046011 |
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| 基金项目： | 山西省农业科学院特色农业技术攻关项目(YGG17021);山西省农业科学院农业科技创新项目(YCX2019T05);国家现代农业技术体系项目(CARS-06-13.5-A23);国家农业环境数据中心观测检测任务(ZX03S0410)共同资助。 |
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| 摘 要： | 通过序列比对在谷子基因组中鉴定出一个PPDK基因,命名为SiPPDK1.为揭示该基因对逆境胁迫的响应,本研究通过对SiPPDK1的基因结构、蛋白特征、功能、启动子区域顺势元件、亚细胞定位、进化特征等进行了系统的分析和预测.荧光实时定量PCR检测了该基因在苗期不同逆境、关键生育期干旱以及不同光照条件下的表达.结果表明该基...
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| 关 键 词： | 谷子 丙酮酸磷酸双激酶 非生物逆境 基因表达 |
Response of Pyruvate Orthophosphate Dikinase(SiPPDK1) to Abiotic Stresses in Foxtail Millet |
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| Du Yanwei,Wang Gaohong,Li Yanfang,Zhao Genyou,Yan Xiaoguang,Wang Zhenhua,Wang Yuwen,Yu Aili,Zhao Jinfeng. Response of Pyruvate Orthophosphate Dikinase(SiPPDK1) to Abiotic Stresses in Foxtail Millet[J]. Molecular Plant Breeding, 2021, 19(1): 13-21 |
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| Authors: | Du Yanwei Wang Gaohong Li Yanfang Zhao Genyou Yan Xiaoguang Wang Zhenhua Wang Yuwen Yu Aili Zhao Jinfeng |
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| Affiliation: | (Shanxi Key Laboratory of Genetic Resources and Breeding in Minor Crops,Millet Research Institute,Shanxi Academy of Agricultural Sciences,Changzhi,046011) |
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| Abstract: | A PPDK gene named Si PPDK1 was identified in foxtail millet genome by sequence alignment.To reveal the response of Si PPDK1 to abiotic stress,the gene structure,protein characteristics,function,cis-elements,subcellular localization predictions and evolution of Si PPDK1 were analyzed and predicted by online tools and software.The quantitative real-time PCR(q RT-PCR)was used to detect the induced expression of Si PPDK1 under different stresses during seedling stage and under drought during key growth stages.The results showed that Si PPDK1 was located on chromosome 9 in the foxtail millet genome,containing 17 introns and 2 alternative transcripts.Functional domain analysis and multiple sequence alignment revealed that the Si PPDK1 protein has a very conserved sequence structure and is very similar to other plant PPDK proteins.q RT-PCR analysis showed that the expression of Si PPDK1 was strongly up-regulated under PEG,ABA,Na Cl and low temperature at its seedling stage.Further studies indicated that Si PPDK1 was involved in drought stress and different light intensity in jointing,heading and filling stage.It is speculated that Si PPDK1 is involved in abiotic stress,especially plays an important role under drought and light stresses at the heading stage. |
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| Keywords: | Foxtail millet Pyruvate orthophosphate dikinase(PPDK) Abiotic stress Gene expression |
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