| 流式细胞术测定5种苔藓植物的基因组大小 |
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| 引用本文： | 李青,普晓俊,马文章,李萍,王继华,王跃华,刘莉. 流式细胞术测定5种苔藓植物的基因组大小[J]. 分子植物育种, 2021, 0(4): 1121-1127 |
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| 作者姓名： | 李青 普晓俊 马文章 李萍 王继华 王跃华 刘莉 |
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| 作者单位： | 云南大学生命科学学院;中国科学院昆明植物研究所;云南省农业科学院花卉所;湖北大学生命科学院 |
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| 基金项目： | 国家自然科学基金项目(31971410)资助。 |
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| 摘 要： | 作为藻类和维管植物之间的过渡类群,苔藓植物不仅占有重要的进化地位,它同时可作为园艺上的观赏植物.目前对很多苔藓植物的培养条件、基因组大小等信息非常缺乏,且已进行基因组测序的苔藓物种较少,这些都严重制约了苔藓植物系统学的研究和资源利用.本研究以基因组大小为942Mb的野生番茄(Solanum pennellii)为内标...
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| 关 键 词： | 苔藓 流式细胞术 基因组大小 |
Determination of Genome Size from Five Moss Species by Flow Cytometry |
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| Li Qing,Pu Xiaojun,Ma Wenzhang,Li Ping,Wang Jihua,Wang Yuehua,Liu Li. Determination of Genome Size from Five Moss Species by Flow Cytometry[J]. Molecular Plant Breeding, 2021, 0(4): 1121-1127 |
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| Authors: | Li Qing Pu Xiaojun Ma Wenzhang Li Ping Wang Jihua Wang Yuehua Liu Li |
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| Affiliation: | (School of Life Sciences,Yunnan University,Kunming,650091;Kunming Institute of Botany,Chinese Academy of Sciences,Kunming,650201;Flower Research Institute,Yunan Academy of Agricultural Sciences,Kunming,650205;School of Life Sciences,Hubei University,Wuhan,430062) |
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| Abstract: | Being transit plant group that bridges the gap between aquatic green algae and terrestrial flowering plants,bryophytes not only play important roles in understanding land plant evolution,but they are also of great aesthetic and ornamental value in horticultural research.Currently,only few bryophyte genomes have been sequenced,and the information on cultural conditions and genome size are missing for many mosses,which hinders the development and utilization of bryophyte resources.In this study,the wild tomato species Solanum pennellii which genome size is 942 Mb was used as internal standard to determine the genome size of five moss species.The results showed that the genome sizes were 391.40 Mb for Brachymeniopsis gymnostoma,386.96 Mb for Brachythecium velutinum,441.01 Mbp for Entosthodon wichurae,493.21 Mb for Leucobryum juniperoideum and 337.94 Mb for Vesicularia montagnei.Our results provide preliminary insights for the whole genome sequencing,molecular biology,phylogenetic evolution,and the development and breeding of horticultural bryophytes. |
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| Keywords: | Bryophyte Flow cytometry Genome size |
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