| 四川地区华重楼(Paris polyphylla var.chinensis)主要病害调查及病原菌鉴定 |
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| 引用本文： | 伏荣桃,王剑,陈诚,克永霞,陈雪娟,卢代华. 四川地区华重楼(Paris polyphylla var.chinensis)主要病害调查及病原菌鉴定[J]. 分子植物育种, 2021, 0(1): 209-217 |
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| 作者姓名： | 伏荣桃 王剑 陈诚 克永霞 陈雪娟 卢代华 |
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| 作者单位： | 四川省农业科学院植物保护研究所;四川省农业科学院;西南民族大学药学院 |
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| 基金项目： | 四川省科技计划项目应用基础研究(2018JY0111);四川省科技计划项目(2016NZ0032)共同资助。 |
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| 摘 要： | 华重楼是重要的中药材,近年来,华重楼病害己成为制约四川华重楼产量和品质提高的重要因素之一.本研究对四川种植区华重楼病害进行了调查,共调查到主要危害华重楼病害有4种.通过组织分离法对相应的病原菌进行分离,并对病原菌进行致病性测定、形态学观察和分子生物学鉴定.试验结果显示,结合病原菌形态特征及rDNA-ITS、HSP60、...
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| 关 键 词： | 华重楼(Paris polyphylla var.chinensis) 病害调查 病原菌鉴定 |
Fungal Disease Survey and Pathogen Identification on Paris polyphylla var.chinensis in Sichuan |
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| Fu Rongtao,Wang Jian,Chen Cheng,Ke Yongxia,Chen Xuejuan,Lu Daihua. Fungal Disease Survey and Pathogen Identification on Paris polyphylla var.chinensis in Sichuan[J]. Molecular Plant Breeding, 2021, 0(1): 209-217 |
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| Authors: | Fu Rongtao Wang Jian Chen Cheng Ke Yongxia Chen Xuejuan Lu Daihua |
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| Affiliation: | (Institute of Plant Protection,Sichuan Academy of Agricultural Science,Chengdu,610066;Key Laboratory of Integrated Pest Management on Crops in Southwest,Ministry of Agriculture Science,Sichuan Academy of Agricultural Science,Chengdu,610066;College of Pharmacy,Southwest Minzu University,Chengdu,610041) |
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| Abstract: | Paris polyphylla var.chinensis is an important Chinese medicinal herb.In recent years,the disease has become one of the important factors restricting the improvement of yield and quality.In this study,fungal diseases of P.polyphylla var.chinensis were surveyed in Sichuan planting areas,and found that there were main four kinds of fungal disease on P.polyphylla var.chinensis.The pathogenic fungus was isolated by using tissue separation,and the pathogen was identified with methods of pathogenicity test,morphological observation and molecular biological analysis.Based on the morphological characteristics and the analysis of the gene sequences of r DNA-ITS,HSP60,G3 PDH,RPB2,TEF,ACT,LSU,Altα1 and TUB,and building the molecular phylogenetic tree,the four fungal disease caused by Epicoccum sorghinu,Alternaria tenuissima,Botrytis cinerea(gray mold),Alternaria alternate,respectively.At present,the most serious diseases were gray mold,brown leaf spot and leaf blight.Fungal disease survey and pathogens identification of P.polyphylla var.chinensis can provide a theoretical basis for the prevention and control of the disease. |
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| Keywords: | Paris polyphylla var.chinensis Disease investigation Pathogen identification |
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