基于高通量测序的不同轮作方式下烟田土壤细菌群落组成分析

在自然界中,土壤微生物与植物之间存在着密切的联系,土壤微生物依赖植物提供的养分而生存,同时对植物的生长发育起着促进作用.本研究利用Illumina HiSeq高通量测序技术,提取西双版纳烟田烟草连作、田菁轮作和青蒿轮作3种种植模式下土壤样品的总DNA,通过PCR方法扩增构建16S rRNA基因文库.结合生物信息学方法分...

Analysis of Bacterial Community Composition in Tobacco Field under Different Rotation Patterns Based on High-Throughput Sequencing

In nature,there is a close relationship between soil microbes and plants.Soil microbes depend on the nutrients provided byplants to survive,and at the same time,play a role in promoting plant growth and development.In this study,Illumina Hi Seq high-throughput sequencing technology was used to extract the total DNA of soil samples from three cropping models of tobacco continuous cropping,Sesbania rotation and Artemisia rotation in Xishuangbanna,and the 16 S r RNA gene library was amplified by PCR(Polymerase chain reaction).Combined with bioinformatics methods,the diversity index and community structure of soil bacteria 16 S r RNA gene V3~V4 region were analyzed to determine the better planting pattern in tobacco fields.Through high-throughput sequenced and analyzed,a total of 4898 bacterial taxonomic units were obtained.The alpha diversity and beta diversity analysis showed that there were significant differences in bacterial community diversity under the three treatments,and the bacterial community richness was the highest in the control group,while the lowest in the Artemisia rotation.The dominant species in the sample were Proteobacteria,Acidobacteria,Bacteroidetes and Chloroflexi,and the relative abundance of Proteobacteria was 33.49%.The dominant genus were Nitrospira,Kaistobacter,Rhodoplanes and Flavobacterium.Nitrospira had the highest relative abundance in Sesbania rotation,which promotes nitrogen conversion.The genus Ralstonia had the lowest relative abundance in Artemisia rotation,which could cause bacterial wilt.Pseudomonas was the most abundant in Artemisia rotation,which could antagonize black shank disease.The results of this study will provide molecular support and theoretical basis for tobacco crop rotation and crop rotation selection.