| 大豆KTI1基因表达方式分析及其启动子预测 |
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| 引用本文： | 赵艳,王珏,刘阔成,孙天国. 大豆KTI1基因表达方式分析及其启动子预测[J]. 分子植物育种, 2021, 19(2): 380-384 |
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| 作者姓名： | 赵艳 王珏 刘阔成 孙天国 |
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| 作者单位： | 齐齐哈尔大学生命科学与农林学院,抗性基因工程与寒地生物多样性保护黑龙江省重点实验室,齐齐哈尔,161006;齐齐哈尔大学生命科学与农林学院,抗性基因工程与寒地生物多样性保护黑龙江省重点实验室,齐齐哈尔,161006;齐齐哈尔大学生命科学与农林学院,抗性基因工程与寒地生物多样性保护黑龙江省重点实验室,齐齐哈尔,161006;齐齐哈尔大学生命科学与农林学院,抗性基因工程与寒地生物多样性保护黑龙江省重点实验室,齐齐哈尔,161006 |
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| 基金项目： | 齐齐哈尔大学研究生创新科研项目(YJSCX2019052)资助。 |
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| 摘 要： | 为明确大豆胰蛋白酶抑制剂基因(KTI1)在不同逆境、ABA条件下及大豆不同组织中的表达方式,本研究采用实时荧光定量PCR技术检测,结果表明KTI1基因受低温、盐、干旱和ABA诱导,分别在处理10、5、24和lh时获得诱导最高值,相对表达量分别是未处理的288.65,274.35,136.04和56.48倍;KTI1基因...
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| 关 键 词： | 大豆 KTI1基因 启动子 |
Analysis of Expression Patterns of KTI1 Gene in Soybean and Its Promoter Prediction |
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| Zhao Yan,Wang Jue,Liu Kuocheng,Sun Tianguo. Analysis of Expression Patterns of KTI1 Gene in Soybean and Its Promoter Prediction[J]. Molecular Plant Breeding, 2021, 19(2): 380-384 |
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| Authors: | Zhao Yan Wang Jue Liu Kuocheng Sun Tianguo |
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| Affiliation: | (Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas,College of Life Science and Agroforestry,Qiqihar University,Qiqihar,161006) |
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| Abstract: | In order to clarify the expression pattern of soybean trypsin inhibitor gene(KTI1)in different stress,ABA conditions and different tissues of soybean,the Quantitative Real-Time PCR method was used in this study.The results showed that KTI1 gene was induced by low temperature,salt,drought and ABA,inducing high respectively in the treatment of 10,5,24 and 1 h.The relative expression levels were 288.65,274.35,136.04 and 56.48 times higher than that of untreated.The expression of soybean KTI1 gene in soybean seeds was relatively high,and when the expression level was 1 in leaves,the expression levels in seeds was 8.70.According to soybean genome sequence,1831 bp upstream promoter sequence of KTI1 gene was amplified.Bioinformatics predictive analysis showed that there were a variety of cis-regulatory elements in promoter that often appeared in stressinduced promoters.It was speculated that soybean KTI1 gene promoter might induce by low temperature,salt,drought and ABA.This study provides a theoretical foundation for further study and application of soybean KTI1 gene and its promoter. |
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| Keywords: | Soybean KTI1 gene Promoter |
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