| 高产优质抗病棉花品种中棉所12号细菌人工染色体(BAC)文库构建 |
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| 引用本文： | 郑拥民,王省芬,张桂寅,李喜焕,马峙英. 高产优质抗病棉花品种中棉所12号细菌人工染色体(BAC)文库构建[J]. 河北农业大学学报, 2004, 27(3): 17-20 |
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| 作者姓名： | 郑拥民 王省芬 张桂寅 李喜焕 马峙英 |
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| 作者单位： | 河北农业大学,农学院,河北省作物种质资源重点实验室,河北,保定,071001 |
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| 基金项目： | 国家自然科学基金(30270848)，教育部重点科技项目(204017) |
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| 摘 要： | 中棉所12号是我国自育的高产、优质、抗枯萎病、耐黄萎病棉花品种，其重要创新是使抗性和产量、品质得到结合改良和提高。本研究以plndigoBAC-5为载体，对中棉所12号进行了细菌人工染色体(BAC)文库构建。初步建立的文库含有38800个克隆，覆盖2倍基因组。对文库中132个重组克隆的分析表明：插入片段为50～150kb，平均大小为120kb；大于100kb的克隆占87．7％，大于110kb的克隆占56％；空载率小于1％。该文库的构建为深入开展棉花基因组有关研究奠定了基础。
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| 关 键 词： | 棉花 中棉所12号 细菌人工染色体 文库构建 |
| 文章编号： | 1000-1573(2004)03-0017-04 |
BAC library construction of Zhongmiansuo 12 with high yield, high quality and disease resistance |
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| ZHENG Yong-min,WANG Xing-fen,ZHANG Gui-yin,LI Xi-huan,MA Zhi-ying sources of Hebei,College of Agriculture,Agricultural University of Hebei,Baoding ,China). BAC library construction of Zhongmiansuo 12 with high yield, high quality and disease resistance[J]. Journal of Agricultural University of Hebei, 2004, 27(3): 17-20 |
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| Authors: | ZHENG Yong-min WANG Xing-fen ZHANG Gui-yin LI Xi-huan MA Zhi-ying sources of Hebei College of Agriculture Agricultural University of Hebei Baoding China) |
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| Affiliation: | ZHENG Yong-min,WANG Xing-fen,ZHANG Gui-yin,LI Xi-huan,MA Zhi-ying sources of Hebei,College of Agriculture,Agricultural University of Hebei,Baoding 071001,China) |
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| Abstract: | The bacterial artificial chromosome(BAC) library of Zhongmiansuo 12, a Chinese elite cotton variety with high yield, high quality and disease resistance, was constructed based on plndigoBAC-5 vector. The library included 38 800 clones and was two haploid genome equivalents based on a AD genome size of 2 240 Mb. Analysis results of 132 recombinants indicated that the insert DNA size ranged from 50 to 150 kb, averaged 120 kb. As much as 87.7% clones had inserts over 100 kb, and 56% clones over 110 kb. The empty clone was less than 1% in the library. This library provided a useful tool for the cotton genome research. |
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| Keywords: | cotton Zhongmiansuo 12 bacterial artificial chromosome (BAC) construction of library |
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