冬春季温室切段扦插快繁马铃薯脱毒苗

在近几年试验研究的基础上,提出冬春季温室切段扦插快繁马铃薯脱毒苗模式.试验结果表明,温室温度25~28℃,相对湿度90%,光照5000~10000 lx,脱毒苗切段长2.5~3 cm,蘸生根液NAA 20 mg/L+KT 2 mg/L+GA30.5 mg/L,扦插密度1000~1300株/m2,扦插后喷雾生长调节液(NAA 5 mg/L+6-BA 0.5 mg/L+GA31 mg/L)和1/2 MS营养液或1/2 K5营养液+植物叶面肥,20~35 d后,苗高8~10 cm、茎节6~7个时,进行继代切段繁殖,如此重复继代切段繁殖3~4次,脱毒苗增殖20~25倍.

Rapid Propagation of Potato Virus-free Plantlets by Cutting Cuttage in Greenhouse in Winter and Spring

On the basis of the previous experiment results in recent years, the article studied the rapid propagation system of virus-free potato plantlets by cutting euttage in greenhouse in winter and spring. The results showed that the appropriate conditions for cutting cuttage and rapid propagation of potato virus-free plantlets were as follows： the temperature was 25- 28℃, with relative humidity of 90%, light intensity of 5000-10 000lx in greenhouse, cuttings length of 2.5-3 era, density of 1 000-1 300 euttings/m2, and the rooting medium was NAA 20 Ing/L＋KT 2 mg/L ＋GA3 0.5 mg/L. The cutting seedlings, sprayed with the growth regulator solution （NAA 5 mg/L＋6-BA 0.5 mg/L＋GA3 1 rag/L） and 1/2 MS nutrient solution or 1/2 K5 nutrient solution＋foliage fertilizer （Huashuo 828）, took roots and germinated very well. After 20-35 days of growth, the seedlings with height of 8-10 cm and 6-7 nodes were used as basic seedlings for the following subculture cutting euttage and breeding. Finally, the virus-free plantlets proliferated 20-25 times through 3-4 subculture cycles of cutting cuttage propagation.