BDE3胁迫对翡翠贻贝（Perna viridis）SOD、MDA和GSH的影响

采用半静态试验方法，研究不同质量浓度的一溴联苯醚（BDE3）（1 μg·L-1、10 μg·L-1和100 μg·L-1）胁迫1 d、3 d、7d和15 d且清洁海水释放3 d和7 d后，翡翠贻贝（Perna viridis）外套膜和内脏团超氧化物歧化酶（SOD）活性、丙二醛（MDA）质量摩尔浓度和谷胱甘肽（GSH）质量分数的变化规律。结果表明，BDE3胁迫后低浓度组翡翠贻贝两组织SOD活性均受到诱导（P〈0.01），诱导率随胁迫时间延长而下降，中、高浓度组外套膜对BDE3响应比内脏团灵敏；BDE3对翡翠贻贝外套膜b（MDA）的影响总体呈诱导后抑制趋势，对内脏团b（MDA）的影响表现抑制-诱导反复变化，其中低浓度组第3天时诱导率最高（26.04%），高浓度组第7天时抑制率最高（14.92%）；翡翠贻贝外套膜w（GSH）在（BDE3）迫下，低浓度组一直被诱导（P〈0.01），中、高浓度组总体呈诱导后抑制作用，内脏团中高浓度组w（GSH）胁迫第1天受到显著抑制（P〈0.05），随暴露时间的延长，低浓度组w（GSH）显著增加（P〈0.01），中、高浓度组出现诱导抑制的不规律变化。释放阶段结束后仅个别浓度组恢复至对照组水平。

Effects of BDE3 on SOD, MDA and GSH of Perna viridis

To study the variation trends of superoxide dismutase （SOD） activity, malondialdehyde （MDA） level and glutathione （GSH） content in tissues of mantle and visceral mass of Perna viridis, we performed an experiment under semi-static conditions by exposing the samples to BDE3 （1 μg·L-1, 10 μg·L-1 and 100 μg·L-1） for 1 d, 3 d, 7 d and 15 d then releasing them by clean seawater for 3 d and 7 d. The results show that during the exposing period, SOD activities of the 2 tissues in 1 μg·L-1 group were induced very significantly （P〈0.01）, and the induction rate decreased with xposing time; SOD activity of mantle in groups of 10 μg·L-1 and 100 μg·L-1 were more sensible than those of visceral mass. MDA level increased at first then inhibited later in mantle, while induction and inhibition alternated in visceral mass; for 1 μg·L-1 group, the highest induction rate reached 26.04% on 3rd day of exposing while the highest inhibitory rate reached 14.92% on 7th day. GSH content in mantle in 1 μg·L-1 group was induced significantly （P〈0.01） all the time, while it increased at first then inhibited later for groups of 10 μg·L-1 and 100 μg·L-1; in visceral mass, GSH content in 100 μg·L-1 group was induced significantly （P〈0.01） on 1st day, while that in 1 μg·L-1 group increased significantly with exposing time （P〈0.01）; groups of 10 μg·L-1 and 100 μg·L-1 showed irregular trends of induction-inhibition. After the releasing period, only individual groups recovered to the same level in the control.