葛根提取物对不孕奶牛人工诱导泌乳效果的影响

【目的】研究葛根提取物对不孕奶牛人工诱导泌乳效果的影响。【方法】选用10头人工诱导泌乳前产奶量、胎次、体质量相近，空怀没有繁殖疾病的干奶牛，按照配对方法随机分为对照组和试验组。对照组和试验组奶牛饲喂相同基础日粮，试验组奶牛补饲500 mg/（kg·d）的葛根提取物，30 d后对2组奶牛同时进行人工诱导泌乳。在试验第0（补饲葛根提取物前），10，20和30天，采集奶牛血样，分离血浆，测定血浆中雌二醇（E₂）、催乳素（PRL）、生长激素（GH）、胰岛素（INS）、孕酮（P₄）、促卵泡生成素（FSH）和促黄体生成素（LH）的含量，并在诱导泌乳成功后测定其第1、2个月的平均日产奶量和前2个月内的总产奶量。【结果】试验组奶牛血浆中E₂、INS的含量较对照组高，其中在饲喂葛根提取物第30天时，试验组与对照组差异显著（P<0.05）。随着葛根提取物饲喂时间的延长，试验组奶牛血浆PRL、GH含量呈增大趋势，在饲喂第30天时，血浆PRL含量显著高于第0，10和20天（P<0.05），血浆GH含量显著高于第0，10天（P<0.05）。试验组开始产奶的第1个月平均日产奶量较对照组高1.22 kg（P>0.05），第2个月较对照组高2.08 kg（P<0.05）；试验组和对照组在前2个月内个体牛的总产奶量分别为1 592.67 和1 452.31 kg，差异不显著（P>0.05）。【结论】补饲葛根提取物可以增强不孕奶牛人工诱导泌乳的效果，增加产奶量。

Effects of extracts of pueraria labata root on dairy lactation of infertile cows after artificial induction

【Objective】 The research was to study the effects of extracts of pueraria labata root on dairy lactation of infertile cows after artificial induction.【Method】 Ten healthy Holstein cows without pregnancy were selected,which had similar parturition and body weight,and were divided into two groups:control group and experimental group.The feed for control group was basic ration and feed for experimental group was basic ration + 500 mg/(kg·d) extracts of pueraria labata root.After feeding extracts of pueraria labata root,control group and experimental group were artificially inducted to lactation at the same time.In the experiment on 0 day(before the supplement pueraria extract),10,20 and 30 day,the cow blood was collected,plasma separated,the content of plasma E2,PRL,GH,INS,P4,FSH and LH determined;And its average daily milk yield of 1,2 months and the first two months total milk yield in the induction of lactation success were determined.【Result】 In general,the plasma E2 and INS levels of experiment group were higher than that of control group,especially on the 30 d(P<0.05).The plasma PRL and GH levels of experiment group rose with the time going,the plasma PRL,GH levels on the 30 d rose significantly compared with that on the 0,10 and 20 d(P<0.05).The average milk yield of experiment group was 1.22 kg/d more than that of control group in the first month when lactation began(P>0.05),and 2.08 kg/d more in the two months(P<0.05);The total milk yields of experimental group and control group was 1 592.67 and 1 452.31 kg respectively(P>0.05).【Conclusion】 Supplementary of extracts of pueraria labata root could enhance the effects of artificial inducting of lactation for infertile cows,and could significantly increase the milk yield.