| 不同地区海兰褐蛋鸡中J亚群-禽白血病病毒株gp85基因的分子演化分析 |
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| 引用本文： | 代阳,杨其峰,王波,刘绍琼,王秀臻,柴家前,崔治中,孙淑红.不同地区海兰褐蛋鸡中J亚群-禽白血病病毒株gp85基因的分子演化分析[J].畜牧兽医学报,2010,41(5). |
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| 作者姓名： | 代阳 杨其峰 王波 刘绍琼 王秀臻 柴家前 崔治中 孙淑红 |
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| 作者单位： | 山东农业大学动物医学院,泰安,271018 |
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| 基金项目： | 国家公益性行业(农业)科研专项，山东省中青年科学家基金，山东农业大学青年科技创新基金 |
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| 摘 要： | 本研究通过对不同地区海兰褐蛋鸡群中分离的5株J亚群-禽白血病病毒(ALV-J)的囊膜糖蛋白基因(gp85)进行同源性分析,阐述了不同海兰褐鸡群中存在的ALV-J的分子演化规律。对2008-2009年分别从北京、陕西、山东泰安、济阳、曲阜等不同地区饲养的海兰褐鸡分离到的5株ALV-J,用PCR方法克隆gp85基因、测序,并与国内外已发表的14株ALV-Jgp85基因进行同源性比较。结果表明,5株ALV-J与来自白羽肉鸡的HPRS-103株的同源性最近,平均为96.6%(96.4%～96.8%);与来自国内海兰灰蛋鸡的SD07LK1株的同源性平均仅为89.6%(89.3%～89.9%);而5株ALV-J间的同源性高达98.1%以上(98.1%～100%)。本研究发现,不同地区的海兰褐蛋鸡中广泛存在的ALV-J可能有一个共同的来源,即国外的白羽肉鸡。
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| 关 键 词： | 海兰褐蛋鸡 J亚群-禽白血病病毒 gp85基因 同源性 |
Molecular Evolution Analysis for gp85 Gene of Subgroup J-Avian Leukosis Viruses Strains from Hyline Egg-type Chickens in Different Areas |
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| DAI Yang,YANG Qi-feng,WANG Bo,LIU Shao-qiong,WANG Xiu-zhen,CHAI Jia-qian,CUI Zhi-zhong,SUN Shu-hong.Molecular Evolution Analysis for gp85 Gene of Subgroup J-Avian Leukosis Viruses Strains from Hyline Egg-type Chickens in Different Areas[J].Acta Veterinaria et Zootechnica Sinica,2010,41(5). |
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| Authors: | DAI Yang YANG Qi-feng WANG Bo LIU Shao-qiong WANG Xiu-zhen CHAI Jia-qian CUI Zhi-zhong SUN Shu-hong |
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| Institution: | DAI Yang,YANG Qi-feng,WANG Bo,LIU Shao-qiong,WANG Xiu-zhen,CHAI Jia-qian,CUI Zhi-zhong,SUN Shu-hong(College of Animal Science , College of Veterinary Medicine,Sh,ong Agricultural University,Taian 271018,China) |
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| Abstract: | By the homology analysis for gp85 genes of 5 strains of ALV-J isolated from Hyline laying hens,the molecular evolution of ALV-J was studied in the present study.The 5 strains of ALV-J were obtained from Beijing,Shanxi and Shandong.The gp85 genes were cloned by PCR and the sequence was measured and compared with 14 published reference ALV-J strains.The homology analysis showed that the 5 isolated strains of ALV-J shared 96.6%(96.4%-96.8%) and 89.6%(89.3%-89.9%) homology with the HPRS-103 strain isolated from... |
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| Keywords: | Hyline egg-type chickens subgroup J-avian leukosis viruses gp85 gene homology |
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