| 银染mRNA差异显示法与放射自显影法的比较 |
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| 引用本文： | 刘肇宁,爱民. 银染mRNA差异显示法与放射自显影法的比较[J]. 内蒙古农业大学学报(自然科学版), 2008, 29(4) |
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| 作者姓名： | 刘肇宁 爱民 |
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| 作者单位： | 内蒙古医学院,呼和浩特市,010059;内蒙古医学院,呼和浩特市,010059 |
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| 摘 要： | 目的:建立银染法mRNA差异显示,并与放射自显影法进行比较。方法:以大蒜提取物大蒜素处理胃癌细胞系BGC823细胞,分别进行银染法和放射自显影法的mRNA差异显示,对两种方法所得差异片段进行反向Northern杂交,比较结果。结果:由两种方法得到的片段,杂交后结果基本相符。结论:对高丰度差异表达基因筛选,银染法与放射自显影法mRNA差异显示相比更简便易行。
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| 关 键 词： | mRNA差异显示 银染法 放射自显影法 |
COMPARE SILVER STAINED METHOD WITH RADIOAUTOGRAPH METHOD OF mRNA DIFFERENTIAL DISPLAY |
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| LIU Zhao-ning,AI Min. COMPARE SILVER STAINED METHOD WITH RADIOAUTOGRAPH METHOD OF mRNA DIFFERENTIAL DISPLAY[J]. Journal of Inner Mongolia Agricultural University(Natural Science Edition), 2008, 29(4) |
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| Authors: | LIU Zhao-ning AI Min |
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| Abstract: | Objective:To develop silver staining method of mRNA differential display and to compare with radioautograph method.Methods:Gastric mucosa adenocarcinoma cell lines BGC823 cells treated with allitridi that is the extractive of garlic,we isolated and cloned the differentially expressive gene by both silver staining method and radioautograph method of mRNA differential display.Compare cDNA fragments by reverse Northern blot.Results:The results of Northern blot are similarly.Conclusion:The established mRNA diff... |
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| Keywords: | mRNA Differential Display silver staining audioautography |
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