Cloning and characterization of pea apyrases: involvement of PsAPY1 in response to signal molecules from the pea pathogen Mycosphaerella pinodes |
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Authors: | Tomoharu Kawahara Kazuhiro Toyoda Akinori Kiba Akiko Miura Takako Ohgawara Mikihiro Yamamoto Yoshishige Inagaki Yuki Ichinose Tomonori Shiraishi |
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Affiliation: | (1) Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, Okayama 700-8530, Japan, JP |
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Abstract: | Two nucleoside triphosphatase (NTPase) cDNA clones were isolated from a cDNA library of Pisum sativum L., cv. Midoriusui. The genes encoding the cDNAs were designated PsAPY1 and PsAPY2. PsAPY1 included the N-terminal amino acid sequence of an NTPase bound to pea cell wall. The phylogenic analysis indicated that PsAPY1 belongs to an NTPase subfamily responsive to environmental stimuli and that PsAPY2 belongs to a discrete subfamily, the physiological role of which is almost unknown. The adenosine triphosphatase activity of recombinant PsAPY1 was regulated by an elicitor and a suppressor from the pea pathogen Mycosphaerella pinodes. Based on these findings, we discuss the role of NTPases in response to biological stresses. Received: May 27, 2002 / Accepted: July 31, 2002 |
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Keywords: | Apyrases (NTPases) Biological stresses Elicitor Mycosphaerella pinodes Pisum sativum L. Suppressor |
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