| 金叶龟甲冬青袋式组培应用效果试验初报 |
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| 引用本文： | 史云光,朱艳,徐招娣.金叶龟甲冬青袋式组培应用效果试验初报[J].江苏林业科技,2010,37(3):19-21. |
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| 作者姓名： | 史云光 朱艳 徐招娣 |
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| 作者单位： | 1. 江苏农林职业技术学院,江苏,句容,212400
2. 江苏农林职业技术学院绿苑实业总公司,江苏,句容,212400 |
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| 基金项目： | 江苏省科技成果转化专项资金项目"常绿彩叶苗木新品种选育及组培快繁技术开发与推广"(BA2007100)研究内容之一 |
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| 摘 要： | 以金叶龟甲冬青组培分化苗为供试材料,采用常规瓶式培养和袋式培养方式进行增殖、生根培养。结果表明：2种培养方式生产的组培苗生长状况无明显差异,均表现出增殖系数大,生长正常,生根快,根系发达等,生根率达92%,且苗木健壮,质量好,移栽成活率高（85%以上）。但在培养基灭菌和接种效率、污染率、移植成活率、培养空间利用率和炼苗效率等方面,袋式培养优于常规瓶式培养,可提高生产效率,达到节能增效目的。
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| 关 键 词： | 金叶龟甲冬青 袋式组织培养 效果 |
Effect of bag-type application on the tissue culture of Ilex crenata 'Golden Gem' |
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| SHI Yun-guang,ZHU Yan,XU Zhao-di.Effect of bag-type application on the tissue culture of Ilex crenata 'Golden Gem'[J].Journal of Jiangsu Forestry Science & Technology,2010,37(3):19-21. |
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| Authors: | SHI Yun-guang ZHU Yan XU Zhao-di |
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| Institution: | 1.Jiangsu Polytechnic College of Agriculture and Forestry,Jurong 212400,China;2.Jiangsu Polytechnic College of Agriculture and Forestry Green Palace Co Ltd,Jurong 212400,China) |
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| Abstract: | In order to test the effect of bag-type application on the tissue culture of Ilex crenata ＇Golden Gem＇,the shoots of Ilex crenata ＇Golden Gem＇ were used by common bottle and bag-type tissue culture.The results showed that there was no significant difference in the shoots appearance,and the shoots from the both methods appeared faster multiplication,high-rooting rate（even reached 92 percent） and high survival rate for transplanting plants（above 85percent）.But bag-type tissue culture was better in culture medium sterilizing,plating efficiency,pollution rates,culture space utilization rate and the efficiency of hardening off than those in the other method.Using the method of bag-type tissue culture,the productivity was raised and more energy could be saved. |
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| Keywords: | Ilex crenatal ＇Golden Gem＇ Bag-type tissue culture Influence |
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