Reduced birth weight,cleft palate and preputial abnormalities in a cloned dog |
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Authors: | Min Jung Kim Hyun Ju Oh Geon A Kim Young Kwang Jo Jin Choi Hye Jin Kim Hee Yeon Choi Hyun Wook Kim Min Cheol Choi Byeong Chun Lee |
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Institution: | 1.Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-742, Republic of Korea;2.Haemaru Referral Animal Hospital, 319 Beon-gil, Hwangsaeul-ro, Bundang-gu, Seongnam, Gyeonggi 463-824, Republic of Korea;3.Department of Veterinary Medical Imaging, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-742, Republic of Korea |
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Abstract: | The aim of the present study was to report a novel developmental abnormality in a cloned dog. A fibroblast cell line was established from an 8-year-old male German shepherd dog. In vivo matured oocytes were retrieved from a large breed dog, and the nucleus was removed from each oocyte. A donor cell was injected into an enucleated oocyte, and the oocyte-cell couplet was fused electrically. After chemical activation, the resulting embryos were transferred into a naturally estrus-synchronized recipient dog, and two cloned pups were delivered by Cesarean section 60 days later. One cloned pup (Clone 1) was healthy, but the other (Clone 2) had a birth weight of only 320 g and cleft palate, failure of preputial closure at the ventral distal part, and persistent penile frenulum. Clone 2 was raised by stomach feeding until Day 40 after birth, where palatoplasty was performed. The abnormalities in external genitalia in Clone 2 resulted in persistent penile extrusion that was surgically corrected. This complex developmental abnormality has not been reported in dogs previously. |
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Keywords: | Developmental abnormality Cleft palate Penile frenulum Preputial closure Persistent penile extrusion Cloning Dog |
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