高效液相色谱法测定池塘底泥中呋喃西林的研究

文章研究了池塘底泥中呋喃西林残留量的提取和检测方法，以上V（甲醇）：V（二氯甲烷）=3：7混合溶剂提取，提取效果较好；比较了振荡提取和超声波辅助加热提取法，后者提取能力较强；优化了高效液相色谱测定的色谱条件，在C18反相色谱柱上分离，梯度洗脱比等度洗脱能获得更好峰形，分离效果更好。以V（甲醇）：V（二氯甲烷）=3：7作为提取溶剂、超声波辅助加热法（35 kHz，强度100%，40 ℃）提取，回收率为80%~95%，相对标准偏差小于10%，检测限达到0.250 μg？kg-1。该方法回收率高、稳定性好、灵敏度高、操作简便。

HPLC determination of nitrofurazone in sediment of aquaculture pond

This paper studies the extraction and detection methods for nitrofurazone residues in sediment of aquaculture pond. The extraction by methanol-methylene chloride （3：7） mixed solvent achieved good effects. We compared oscillation extraction with ultrasonic-assisted heating extraction, finding that the latter extracted more. The HPLC chromatographic conditions had been optimized. Isolated on a C18 reversed-phase column, gradient elution obtained better peaks and better separation than isocratic elution. With methanol-methylene chloride （3：7） as extraction solvent, the ultrasonic-assisted heating method （35 kHz, intensity of 100%, 40 ℃） for extraction obtained 80%~95% recovery, and the relative standard of deviation was less than 10% and the detection limit reached 0.250 μg？kg-1. The user-friendly method has high recovery, good stability and high sensitivity.