| 小黑杨转几丁质酶基因及酶活性 |
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| 引用本文： | 王志英,张福丽,王占斌.小黑杨转几丁质酶基因及酶活性[J].林业科学,2010,46(2). |
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| 作者姓名： | 王志英 张福丽 王占斌 |
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| 作者单位： | 1. 东北林业大学,哈尔滨,150040
2. 东北林业大学,哈尔滨,150040;周口师范学院,周口,466000 |
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| 基金项目： | 黑龙江省攻关项目(GB06B303);;黑龙江省重点基金项目(ZJN04-0101);;哈尔滨科技创新人才专项(2006RFQN005)资助 |
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| 摘 要： | This paper reports a successful transformation of chitinase gene into Populus simonii×P. nigra by Agrobacterium-mediated means. For reducing adventitious buds,the optimal concentration of kanamycin was 40 mg·L~ -1 . Up to 700 mg·L~ -1 of Cefazolin Sodium had no obvious effect on differentiation of leaves. About 20-day-old leaf disc explants were pre-cultured for 3-4 d,then immersed in Agrobacterium suspension for infection for 6-15 min,then co-cultured on non-selection culture medium for 3 d in the dark at ...
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| 关 键 词： | 小黑杨 转化 几丁质酶基因 几丁质酶活性 |
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