| 鸡新城疫病毒新乡株ND-XX10的分离与鉴定 |
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| 引用本文： | 银梅,赵坤,陈桂香,宁红梅,贾文科,王选年.鸡新城疫病毒新乡株ND-XX10的分离与鉴定[J].河南农业科学,2011,40(2):142-145. |
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| 作者姓名： | 银梅 赵坤 陈桂香 宁红梅 贾文科 王选年 |
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| 作者单位： | 1. 河南科技学院,动物科学学院,河南,新乡,453003
2. 河南科技学院,动物科学学院,河南,新乡,453003;新乡学院,河南,新乡,453003 |
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| 基金项目： | 国家"十一五"科技支撑计划重大项目子课题，河南科技学院骨干教师资助项目 |
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| 摘 要： | 2010年初,已免疫过新城疫疫苗的新乡市某鸡场蛋鸡出现以消化道、呼吸道出血为主要症状的疑似新城疫病例.将病料经10日龄SPF鸡胚增殖分离病毒,命名为ND-XX10.提取其病毒基因组RNA,并以此为模板,RT-PCR扩增获得F基因主要功能区(1～631 bp)片段并进行测序.结果显示,ND-XX10毒株F蛋白的裂解位点为...
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| 关 键 词： | 新城疫病毒 F基因 RT-PCR |
Isolation and Identification of Chicken Newcastle Disease Virus Xinxiang Strain |
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| YIN Mei,ZHAO Kun,CHEN Gui-xiang,NING Hong-mei,JIA Wen-ke,WANG Xuan-nian.Isolation and Identification of Chicken Newcastle Disease Virus Xinxiang Strain[J].Journal of Henan Agricultural Sciences,2011,40(2):142-145. |
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| Authors: | YIN Mei ZHAO Kun CHEN Gui-xiang NING Hong-mei JIA Wen-ke WANG Xuan-nian |
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| Institution: | 1,2(1.College of Animal Science,Henan Institute of Science&Technology,Xinxiang 453003,China;2.Xinxiang University,Xinxiang 453003,China) |
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| Abstract: | To isolate field Newcastle disease virus(NDV) stains,10-day-old SPF chicken embryos was inoculated with suspected sample collected from disease chicken.The virus genomic RNA is extracted and the main region of F gene(1-631 bp) fragment was amplified by RT-PCR and identified by DNA sequencing.The results showed that a new isolate,namely ND-XX10,was obtained.The deduced amino acid sequence of the F protein of ND-XX10 isolate at the cleavage site was 112R-R-Q-K-R-F117,which is typically found in virulent NDV isolates.The amino acid residues at position 101 and 121 are K and V,respectively.Phylogenetic analysis showed that the isolate belonged to genotype Ⅶe. |
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| Keywords: | RT-PCR |
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