| 百合查尔酮合成酶(chs)基因的cDNA克隆与分析 |
| |
| 引用本文： | 杨丽,刘雅莉,王跃进.百合查尔酮合成酶(chs)基因的cDNA克隆与分析[J].河南农业科学,2011,40(1):124-126. |
| |
| 作者姓名： | 杨丽 刘雅莉 王跃进 |
| |
| 作者单位： | 1. 信阳农业高等专科学校,河南,信阳,464000
2. 西北农林科技大学,园艺学院,陕西,杨凌,712100 |
| |
| 摘 要： | 根据报道的查尔酮合成酶基因的保守序列,设计了特异简并引物,以东方百合Sorbonne盛开的花瓣总RNA为模板,逆转录合成cDNA第一链,用特异引物对此双链cDNA进行PCR扩增,将扩增后的基因片段回收克隆后,送至上海博亚生物公司测序,目的序列长873bp,与已发表的百合中CHS基因的同源性达到91%,说明克隆的片段属于...
|
| 关 键 词： | 百合 查尔酮合成酶 cDNA 克隆 |
Cloning and Sequencing of Chalcone Synthase Gene cDNA in Lilium |
| |
| YANG Li,LIU Ya-li,WANG Yue-jin.Cloning and Sequencing of Chalcone Synthase Gene cDNA in Lilium[J].Journal of Henan Agricultural Sciences,2011,40(1):124-126. |
| |
| Authors: | YANG Li LIU Ya-li WANG Yue-jin |
| |
| Institution: | 1.Xinyang Agricultural College,Xinyang 464000,China;2.College of Horticulture,Northwest Sci-Tech University of Agriculture and Forestry,Yangling 712100,China) |
| |
| Abstract: | The specific primers synthesized according to conserved regions of reported CHS gene were used to acquire CHS cDNA by RT-PCR.A fragment of about 870bp was obtained and the sequencing results showed that: the fragment length was 873bp and its sequence was over 91% homology to other reported CHS.The Genbank accession number was DQ471950. |
| |
| Keywords: | cDNA |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
