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| 聚丙烯酰胺凝胶电泳快速检测大豆过敏原蛋白Gly m Bd30K缺失方法 | |
| 引用本文： | 王绍东,李远明,刘伟,姜妍,王浩,于佳,李文滨,高桥浩司.聚丙烯酰胺凝胶电泳快速检测大豆过敏原蛋白Gly m Bd30K缺失方法[J].大豆科学,2011,30(4):638-642. |
| 作者姓名： | 王绍东 李远明 刘伟 姜妍 王浩 于佳 李文滨 高桥浩司 |
| 作者单位： | 1. 东北农业大学国家大豆工程技术研究中心,黑龙江哈尔滨150030;独立行政法人/农业食品产业技术综合研究机构/作物科学研究所,茨城筑波305-8518 2. 东北农业大学国家大豆工程技术研究中心,黑龙江哈尔滨,150030 3. 东北农业大学大豆生物学教育部重点实验室,黑龙江哈尔滨,150030 |
| 基金项目： | 哈尔滨市科技创新人才研究专项资金资助项目(2010RFLYN016); 东北农业大学留学回国基金资助项目(2010RCA03); 黑龙江省留学回国基金资助项目(LC2009C25) |
| 摘 要： | 通过对现有SDS-PAGE电泳技术的样本前处理和制胶技术的改良,开发出了一种能够同时清晰鉴别7S球蛋白组分亚基(α',α,β-Subunit)和Gly m Bd 30 K谱带的SDS-PAGE电泳快速检测新技术.利用该方法对PI603570A×0329F6杂交组合F2群体的116个样本进行了7S球蛋白组分亚基(α',α... |
| 关 键 词： | 过敏原蛋白 聚丙烯酰胺凝胶电泳 免疫印迹技术 大豆种子 |
A Fast Detection Method for Screening of Gly m Bd 30 K Allergen Protein Using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis in Soybean Seeds |
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| WANG Shao-dong,LI Yuan-ming,LIU Wei,JIANG Yan,WANG Hao,YU Jia,LI Wen-bin,TAKAHASHI Koji.A Fast Detection Method for Screening of Gly m Bd 30 K Allergen Protein Using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis in Soybean Seeds[J].Soybean Science,2011,30(4):638-642. | |
| Authors: | WANG Shao-dong LI Yuan-ming LIU Wei JIANG Yan WANG Hao YU Jia LI Wen-bin TAKAHASHI Koji |
| Institution: | WANG Shao-dong1,3,LI Yuan-ming1,LIU Wei1,JIANG Yan1,WANG Hao1,YU Jia1,LI Wen-bin2,TAKAHASHI Koji3(1.National Research Center of Soybean Engineering and Technology(NRCSET),Northeast Agricultural University,Harbin 150030,Heilongjiang,China,2.Key Laboratory of Soybean Biology of Chinese Education Ministry,3.National Agriculture and Food Research Organization,National Institute of Crop Science,Tsukuba 305-8518,Ibaraki,Japan) |
| Abstract: | In this research,through the improvement of the sample pretreatment and confection of the gel,a fast detection method was developed for simultaneous determination of α’,α,β-Subunit and Gly m Bd 30 K lacking individual using sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE)in soybean seeds.We have screened F2(PI603570A×0329F6)population(N=116)in 7S globuline lacking by this method.Moreover,we have introduced the lacking character into the major soybean varieties in Heilongjiang province through the backcross breeding.The results indicated that the method was better in velocity and less costs of the screening.It is significant for the development of soybean germplasm in the allergen proteins lacking. |
| Keywords: | Allergen proteins Sodium dodecyl sulfate polyacrylamide gel electrophoresis Western blotting Soybean seeds |
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