| 大豆异黄酮合成关键酶基因的克隆及表达分析 |
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| 引用本文： | 钱丹丹,龚德顺,焦丽,翟莹,张海军,李景文,王英,王庆钰.大豆异黄酮合成关键酶基因的克隆及表达分析[J].大豆科学,2011,30(5):743-748. |
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| 作者姓名： | 钱丹丹 龚德顺 焦丽 翟莹 张海军 李景文 王英 王庆钰 |
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| 作者单位： | 1. 吉林大学植物科学学院,植物种质资源与利用实验室,吉林长春130062
2. 中国科学技术大学生命科学学院,合肥微尺度物质科学国家实验室,安徽合肥230027 |
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| 基金项目： | 转基因生物新品种培育重大专项资助项目(2008ZX08004-003); 吉林省科技厅重点资助项目(20080204); 国家自然科学基金资助项目(30971808) |
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| 摘 要： | 采用RT-PCR法克隆了大豆异黄酮生物合成途径中查尔酮合酶(Chalcone synthase,CHS)和查尔酮异构酶(Chalcone Isomerase,CHI)2个关键酶基因,并利用SOE-PCR技术成功将2个基因融合,将其均构建成原核表达载体,在大肠杆菌中进行了初步的表达研究,为进一步研究CHS和CHI 2种关...
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| 关 键 词： | 大豆异黄酮 查尔酮合酶 查尔酮异构酶 表达分析 |
Cloning and Expression Analysis of Key Enzymes Genes in Biosynthesis of Soybean Isoflavones |
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| QIAN Dan-dan,GONG De-shun,JIAO Li,ZHAI Ying,ZHANG Hai-jun,LI Jing-wen,WANG Ying,WANG Qing-yu.Cloning and Expression Analysis of Key Enzymes Genes in Biosynthesis of Soybean Isoflavones[J].Soybean Science,2011,30(5):743-748. |
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| Authors: | QIAN Dan-dan GONG De-shun JIAO Li ZHAI Ying ZHANG Hai-jun LI Jing-wen WANG Ying WANG Qing-yu |
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| Institution: | QIAN Dan-dan1,GONG De-shun2,JIAO Li1,ZHAI Ying1,ZHANG Hai-jun1,LI Jing-wen1,WANG Ying1,WANG Qing-yu1(1.Plant Germplasm and Application Laboratory,Plant Science College,Jilin University,Changchun 130062,Jilin,2.Hefei National Laboratory for Physical Sciences at Microscale,School of Life Science,University of Science and Technology of China,Hefei 230026,Anhui,China) |
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| Abstract: | Chalcone Synthase(CHS)and Chalcone Isomerase(CHI)are two key enzymes in soybean isoflavones biosynthesis pathway,the cDNAs of CHS and CHI were cloned using RT-PCR method and these two genes were fused using SOE-PCR,then constructed E.coli expression vectors to study the expression.The eukaryocyte expression vectors were also constructed and transferred into soybean cotyledonary nodes using Agrobacterium-mediated transformation,and then obtained the resistant transgenic plants. |
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| Keywords: | Soybean isoflavones Chalcone synthase Chalcone Isomerase Expression Analysis |
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