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| 利用农杆菌介导法将BrCS基因导入大豆的研究 | |
| 引用本文： | 张书利,刘丽君,唐晓飞,魏崃.利用农杆菌介导法将BrCS基因导入大豆的研究[J].大豆科学,2011,30(4):569-573. |
| 作者姓名： | 张书利 刘丽君 唐晓飞 魏崃 |
| 作者单位： | 1. 黑龙江省农业科学院大豆研究所,黑龙江哈尔滨150086;东北农业大学农学院,黑龙江哈尔滨150030 2. 黑龙江省农业科学院大豆研究所,黑龙江哈尔滨,150086 3. 东北农业大学农学院,黑龙江哈尔滨,150030 |
| 基金项目： | 国家转基因重大专项资助项目(2008ZX08004-002); 现代农业产业技术体系资助项目(nycytx-004) |
| 摘 要： | 以大豆子叶节作为外植体,通过农杆菌介导法将含有BrCS基因的pCAMBIA3300的双子叶植物表达载体,导入到大豆品种黑农59和黑农53中,并对黑农59遗传转化的影响因素进行了优化,最终获得了抗性植株.经PCR检测和PCR-Southern杂交分析,初步证明了目的基因已整合到大豆的基因组中. |
| 关 键 词： | 大豆 遗传转化 BrCS基因 抗冷性 耐盐性 |
Transformation of BrCS Gene into Soybean by Agrobacterium-mediated Method |
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| ZHANG Shu-li,LIU Li-jun,TANG Xiao-fei,WEI Lai.Transformation of BrCS Gene into Soybean by Agrobacterium-mediated Method[J].Soybean Science,2011,30(4):569-573. | |
| Authors: | ZHANG Shu-li LIU Li-jun TANG Xiao-fei WEI Lai |
| Institution: | ZHANG Shu-li1,2,LIU Li-jun1,TANG Xiao-fei1,WEI Lai2(1.Soybean Research Institute,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,2.College of Agriculture,Northeast Agricultural University,Harbin 150030 Heilongjiang,China) |
| Abstract: | In this study,plant expression vector pCAMBIA3300-BrCS containing-BrCS-gene was constructed,and transgenic plants were obtained by Agrobacterium-mediated transformation of soybean(Heinong 59 and Heinong53)cotyledonary nodes.We regenerated plants and optimized the conditions of transformation to increase the efficiency of transformation.PCR and PCR-Southern detection was used to identify the integrated level of BrCS gene in transgenic plants;it confirmed the integration of BrCS genes into the genome of soybe... |
| Keywords: | Soybean Genetic transformation BrCS gene Cold-stress tolerance Salt-stress tolerance |
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