| 几种抑制剂和金属离子对桑尺蠖多酚氧化酶活性的影响 |
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| 引用本文： | 张永亮,朱勇.几种抑制剂和金属离子对桑尺蠖多酚氧化酶活性的影响[J].贵州农业科学,2011(9):68-70. |
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| 作者姓名： | 张永亮 朱勇 |
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| 作者单位： | 周口师范学院生命科学系;西南大学生物技术学院; |
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| 基金项目： | 教育部博士学科点专项基金“家蚕Bm-mof基因的克隆与功能研究”(20060635008); 重庆市教委基金项目“家蚕剂量补偿机制的研究”(040208) |
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| 摘 要： | 为给开发环境友好型生物杀虫剂奠定基础,采用分光光度法检测了几种抑制剂和金属离子对桑尺蠖(Phthonandria atrineata)多酚氧化酶活性的影响.结果表明:槲皮素和硫脲对该酶的抑制浓度(IC50)分别为0.036 mmol/L和0.12 mmol/L,Cu2+和Zn2+对该酶有强烈的抑制作用,而Ca2+和Mg...
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| 关 键 词： | 桑尺蠖 多酚氧化酶 抑制剂 金属离子 |
Effects of Several Inhibitors and Metal Ions on the Activity of Polyphenoloxidase from Phthonandria atrineata |
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| ZHANGYong-liang,ZHU Yong.Effects of Several Inhibitors and Metal Ions on the Activity of Polyphenoloxidase from Phthonandria atrineata[J].Guizhou Agricultural Sciences,2011(9):68-70. |
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| Authors: | ZHANGYong-liang ZHU Yong |
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| Institution: | ZHANGYong-liang1,ZHU Yong 2 (1.Department of Life Science,Zhoukou Normal University,Zhoukou,Henan 466001,2.College of Biotechnology,Southwest University,Chongqing 400715,China) |
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| Abstract: | In order to lay the foundation of the developing environment-friendly bio-pesticides,the effects of several inhibitors and metal ions on the activity of polyphenoloxidase(PPO) from Phthonandria atrineata were investigated with spectrophotometry.The results showed that the two compounds could inhibit PPO activity,and the inhibitor concentrations leading to a loss of 50% activity(IC50) were estimated to be 0.036 mmol/L and 0.12 mmol/L,respectively.The activity of PPO was inhibited by Cu2+ and Zn2+,and increas... |
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| Keywords: | Phthonandria atrineata polyphenoloxidase inhibitor metalions |
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