| 大豆籽粒中脂肪酸组分快速检测方法的比较分析 |
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| 引用本文： | 于福宽,孙君明,韩粉霞,葛一楠,张晶莹,马磊,张金巍,闫淑荣,杨华.大豆籽粒中脂肪酸组分快速检测方法的比较分析[J].大豆科学,2011,30(4):626-631. |
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| 作者姓名： | 于福宽 孙君明 韩粉霞 葛一楠 张晶莹 马磊 张金巍 闫淑荣 杨华 |
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| 作者单位： | 中国农业科学院作物科学研究所,国家农作物基因资源与基因改良重大科学工程,北京100081 |
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| 基金项目： | 转基因重大专项资助项目(2008ZX08004-003,2008ZX08004-004); 国家高技术研究发展计划(863计划)资助项目(2006AA100104); 国家科技支撑计划资助项目(2006BAD13B05,2006BAD01A04); 国家自然科学基金资助项目(30000107); 中央公益性科研院所基本科研业务费资助项目(2060302) |
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| 摘 要： | 选取脂肪酸组分含量具有显著差异的5份大豆种质为材料,采用索氏抽提法、改良的籽粒直接甲酯化法和豆粉直接甲酯化法3种方法处理样品,利用气相色谱(GC)技术分析大豆5种脂肪酸棕榈酸、硬脂酸、油酸、亚油酸和亚麻酸的组分含量,并对3种样品处理方法进行比较分析.结果显示籽粒直接甲酯化法简便快捷,适用于低世代的单籽粒或半籽粒的样品量...
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| 关 键 词： | 大豆 脂肪酸 气相色谱法(GC) |
Comparison of Three Rapid Gas Chromatograph(GC)Methods for Determination of Fatty Acid Components in Soybean Seeds |
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| YU Fu-kuan,SUN Jun-ming,HAN Fen-xia,GE Yi-nan,ZHANG Jing-ying,MA Lei,ZHANG Jin-wei,YAN Shu-rong,YANG Hua.Comparison of Three Rapid Gas Chromatograph(GC)Methods for Determination of Fatty Acid Components in Soybean Seeds[J].Soybean Science,2011,30(4):626-631. |
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| Authors: | YU Fu-kuan SUN Jun-ming HAN Fen-xia GE Yi-nan ZHANG Jing-ying MA Lei ZHANG Jin-wei YAN Shu-rong YANG Hua |
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| Institution: | YU Fu-kuan,SUN Jun-ming,HAN Fen-xia,GE Yi-nan,ZHANG Jing-ying,MA Lei,ZHANG Jin-wei,YAN Shu-rong,YANG Hua(Institute of Crop Science,Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement(NFCRI),Beijing 100081,China) |
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| Abstract: | Five kinds of fatty acid components including palmitic acid,stearic acid,oleic acid,linoleic acid,linolenic acid,were analyzed by the gas chromatograph(GC)method,and three sample handling methods,including Soxhlet extraction,improved seed-methyl esterification and powder-methyl esterification methods,were compared in the five soybean varieties in order to reveal the differentiation among them.The results showed that the seed-methyl esterification method is simple and fast,which is suitable for the determina... |
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| Keywords: | Soybean[Glycine max(L )Merr ] Fatty acids Gas chromatography(GC) |
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