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新城疫病毒SXFP株的分离鉴定
引用本文:吴辉,赵战峰,尚文博.新城疫病毒SXFP株的分离鉴定[J].动物医学进展,2008,29(11).
作者姓名:吴辉  赵战峰  尚文博
作者单位:咸阳职业技术学院生物科技系,陕西咸阳,712000
基金项目:咸阳职业技术学院科研基金项目(07A01)
摘    要:从陕西富平某种鸡场发病鸡群中分离出一株流行毒株,经HA—HI试验和动物回归试验,确定分离到的病毒为鸡新城疫病毒(NDV),命名为SXFP株。经过测定。SXFP株鸡胚半数致死量(ELD50)为10^9.0/0.1mL;鸡胚最小致死量的平均致死时间(MDT)为52.3h,对1日龄SPF雏鸡脑内致病指数(ICPI)为1.92,提示SXFP株为强毒株。血清HI交叉反应试验显示SXFP株和La Sota株存在较小的抗原性差异。

关 键 词:新城疫病毒  SXFP毒株  毒力测定

Isolation and Identification of NDV SXFP Strain
WU Hui,ZHAO Zhan-feng,SHANG Wen-bo.Isolation and Identification of NDV SXFP Strain[J].Progress In Veterinary Medicine,2008,29(11).
Authors:WU Hui  ZHAO Zhan-feng  SHANG Wen-bo
Institution:Department of Biotechnology;Xianyang Vocational Technical College;Xianyang;Shaanxi;712000;China
Abstract:A virus strain was isolated from a chicken farm in Fuping,Shaanxi.It was identified as a NDV strain by HA-HI tests and animal regression test,and named as SXFP strain.The ELD50,MDT and ICPI of SXFP strain were 109.0/0.1 mL,52.3 h and 1.92,respectively.The results indicated that SXFP strain was a virulent strain.The seral HI cross-reaction test showed that the antigenicity between SXFP strain and La Sota strain had little difference.
Keywords:Newcastle disease virus  pandemic strain  virulent test  
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