| 长喙田菁植物螯合肽合成酶PCS2的原核表达及纯化 |
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| 引用本文： | 李安明,邓青云,李德华. 长喙田菁植物螯合肽合成酶PCS2的原核表达及纯化[J]. 甘肃农业大学学报, 2011, 46(6): 150-154 |
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| 作者姓名： | 李安明 邓青云 李德华 |
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| 作者单位： | 孝感学院生命科学技术学院,湖北孝感,432000 |
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| 基金项目： | 湖北省重点(培育)学科:农业资源利用，湖北省自然科学基金项目 |
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| 摘 要： | 为了获得纯化的长喙田菁(Sesbania rostrata)植物螯合肽合成酶PCS2,以原核表达载体pMAL-c2x为基础,构建了含有SrPCS2开放阅读框序列的原核表达载体pAM57,将其转化表达菌株BL21 (DE3),对融合蛋白的表达进行了优化,通过Western blotting鉴定融合蛋白,并用麦芽糖亲和柱对...
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| 关 键 词： | 植物螯肽合成酶 SrPCS2 原核表达 纯化 |
Expression and purification of Sesbania rostrata phytochelatin synthase2 in Escherichia coli |
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| LI An-ming,DENG Qing-yun,LI De-hua. Expression and purification of Sesbania rostrata phytochelatin synthase2 in Escherichia coli[J]. Journal of Gansu Agricultural University, 2011, 46(6): 150-154 |
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| Authors: | LI An-ming DENG Qing-yun LI De-hua |
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| Abstract: | The mature peptide of Sesbania rostrata phytochelatin synthase2(SrPCS2) was subcloned into pMAL-c2x vector to construct the prokaryotic expression vector pAM57.The recombinant plasmid was transformed into E.coli BL21(DE3),then the fused protein MBP-SrPCS2 was induced by IPTG at different temperature,identified by Western blotting and purified through Maltose Binding column.The results suggested that the soluble fusion protein MBP-SrPCS2 was expressed at high levels 16 h after 0.2 mmol/L of IPTG induction(15℃) and the purified MBP-SrPCS2 was obtained by affinity chromatography. |
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| Keywords: | phytochelatin synthase SrPCS2 prokaryotic expression purification |
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