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| 2株拮抗菌的抑菌作用及产酶能力初探 | |
| 引用本文： | 田恩琴,常永义,朱建兰,靳小刚,雷成军. 2株拮抗菌的抑菌作用及产酶能力初探[J]. 甘肃农业大学学报, 2011, 46(6): 41-45,51 |
| 作者姓名： | 田恩琴 常永义 朱建兰 靳小刚 雷成军 |
| 作者单位： | 1. 甘肃农业大学草业学院,草业生态系统教育部重点实验室,中-美草地畜牧业可持续发展研究中心,甘肃兰州730070 2. 甘肃农业大学农学院,甘肃兰州,730070 3. 甘肃省静宁县林业局,甘肃静宁743400; |
| 基金项目： | 现代农业产业技术体系专项资金资助(Supported by China Agriculture Research system |
| 摘 要： | 利用从葡萄果穗附生微生物区系分离获得的拮抗细菌BHG和BTZ,对葡萄灰霉病菌(Botrytis cinerea)进行抑菌试验.结果表明:BHG和BTZ与葡萄灰霉病菌对峙培养5d后,均产生明显的抑菌带,抑菌带宽度分别为7和10 mm,培养10 d后,仍然保持稳定的抑菌效果；抗真菌谱的结果表明,BHG、BTZ除对粉红单端孢... |
| 关 键 词： | 葡萄灰霉病菌 对峙培养 胞外酶 拮抗细菌 |
Inhibitory effects of two antagonistic bacterial strains and theirs producing extracellular enzymes ability in vitro |
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| TIAN En-qin,CHANG Yong-yi,ZHU Jian-lan,JIN Xiao-gang,LEI Cheng-jun. Inhibitory effects of two antagonistic bacterial strains and theirs producing extracellular enzymes ability in vitro[J]. Journal of Gansu Agricultural University, 2011, 46(6): 41-45,51 | |
| Authors: | TIAN En-qin CHANG Yong-yi ZHU Jian-lan JIN Xiao-gang LEI Cheng-jun |
| Abstract: | The antagonistic bacterial strains BHG and BTZ was isolated from the microflora of grape cluster.The bacteriostatic effects against Botrytis cinerea were tested.The results showed that after 5 days of culture,two bacteriostatic belts of 7 mm and 10 mm in width were observed on the culture plate and the inhibitory activity maintained for 10 d.Antimicrobial spectrum indicated that the strains BHG and BTZ had a broad antifungal spectrum except Valsa japorica(Trichothecium roseum),which could inhibit hyphal growth of at least 8 pathogens with inhibitive rate ranging from 8.12% to 65.12%.Properties of physiological and biochemical indicated that both BHG and BTZ produced amylase,cellulose and lipase. |
| Keywords: | Botrytis cinerea confrontation culture extracellular enzym antagonistic bacterial |
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