微波辅助提取贯叶连翘总黄酮的工艺研究

[目的]探讨贯叶连翘中总黄酮的微波辅助提取优化工艺条件。[方法] 采用正交试验方法，考察微波功率、提取溶剂浓度、液固比、提取时间对总黄酮含量的影响，确定微波辅助提取贯叶连翘总黄酮的最佳工艺条件，并与超声波提取法、索氏提取法进行比较。[结果] 微波辅助提取贯叶连翘总黄酮的最佳条件为乙醇溶液的体积浓度为70%、液固比为30∶1、微波功率450 W、微波提取时间5 min，在该条件下贯叶连翘总黄酮得率为6.02%。微波辅助提取5 min与索氏法提取4 h、超声波法提取1 h得率相当。[结论] 微波辅助提取法具有快速、高效、节能、选择性好等特点。

Study on Extracting Total Flavonoids from Hypericum perfortum L.with Microwave-assisted Extraction

[Objective] The study aims to discuss the optimum technological conditions of extracting total flavonoids from Hypericum perfortum L.with microwave-assisted extraction.[Method] Microwave power,extraction solvent concentration,liquid-solid ratio,and extraction time are investigated and the appropriate microwave-assisted exteaction conditions are obtained with the orthogonal test method.The microwave-assisted exteaction method is compared with ultrasonic extraction and soxhlet extraction methods.[Result] The optimized conditions of microwave-assisted extraction are as follows: ethanol concentration is 70 %,liquid-solid ratio is 30∶ 1,microwave power is 450 W,and extraction time is 5 minutes.Also the extracting yield of flavoniods from Hypericum perfortum L.is up to 6.02 % under the optimal extraction conditions.The yield of total flavonoids is almost equal to soxhlet extraction method for 4 hours,and ultrasonic extraction method for 1 hours.[Conclusion] The microwave-assisted extraction method is a fast,efficient,energy saving and high-selective.