勋章菊组培快繁技术研究

以勋章菊无菌苗叶片为外植体,以MS为基本培养基,添加不同浓度TDZ、2,4-D、IAA、IBA,研究不同浓度激素及组合对丛生芽诱导、试管苗生根的影响,并进行试管苗练苗和移栽.结果表明:最佳丛生芽诱导培养基为MS+TDZ 0.8mg/L+IBA(0.1～0.5)mg/L,诱导率达到90.0％以上.最佳生根培养基为1/2MS+NAA 0.2mg/L+IBA 0.5 mg/L,生根率达到100.0％.勋章菊的叶片是外植体的较好选择,在短期内能获得大量组培苗,移栽成活率也达到95％以上.

Study on Tissue Culture and Rapid Propagation of Gazania rigens L.

In vitro seedling leaves of Cazania leaves were used as explants,by using MS as minimal medium,adding different concentration of TDZ,2,4-D,IAA,IBA,effect of different concentration hormone and their combination on the induction of adventitious buds,rooting and transplanting,practice of test-tube seedling were studied.The results indicated that optimum medium for induction adventitious buds was MS+TDZ 0.8 mg/L+IBA(0.1～0.5)mg/L,induction rate of more than 90.0%.The best rooting medium was 1/2MS+IBA 0.5 mg/L+NAA 0.2 mg/L,the rooting rate reached 100%.The leaf explants of Cazania was a better choice,in the short term to obtain large amounts of tissue culture seedlings,the transplanting survival rate could reach more than 95%.