| 中国石蒜组培苗体细胞无性系变异的ISSR和SRAP检测 |
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| 引用本文： | 巩檑,杨亚珺,周坚. 中国石蒜组培苗体细胞无性系变异的ISSR和SRAP检测[J]. 北方园艺, 2011, 0(22): 108-112 |
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| 作者姓名： | 巩檑 杨亚珺 周坚 |
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| 作者单位： | 南京林业大学森林资源与环境学院,江苏南京,210037;南京润亚生物科技发展有限公司,江苏南京,210037 |
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| 基金项目： | 南京林业大学2009年优秀博士学位论文创新基金资助项目(163010079);江苏省教育厅推广资助项目(JH07-012);镇江市科技局资助项目(NY2008034) |
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| 摘 要： | 以中国石蒜离体培养再生的幼苗为试材,首次采用ISSR和SRAP分子标记对再生苗的遗传稳定性进行了检测,以探讨体细胞无性系变异情况及成因.结果表明:所选用的14条ISSR引物共产生191条带,其中只有1条为多态性条带,并且该条带表现为新增条带；SRAP标记所选用的15对引物共扩增出219条带,其中3条为多态性条带,表现为共有条带缺失.综合2种分子标记检测结果来看,再生植株表现出的体细胞变异率极低(0.97％).现有数据同时也表明,已建立的离体培养体系在保存中国石蒜种质资源和遗传改良上是稳定、可行的.
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| 关 键 词： | 中国石蒜 离体培养 ISSR SRAP 体细胞无性系变异 |
Detection of Somaclonal Variation in Micropropagated Plants of Lycoris chinensis by ISSR and SRAP Markers |
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| GONG Lei , YANG Ya-iun , ZHOU Jian. Detection of Somaclonal Variation in Micropropagated Plants of Lycoris chinensis by ISSR and SRAP Markers[J]. Northern Horticulture, 2011, 0(22): 108-112 |
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| Authors: | GONG Lei YANG Ya-iun ZHOU Jian |
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| Affiliation: | GONG Lei1,YANG Ya-jun2,ZHOU Jian1(1.College of Forest Resources and Environment,Nanjing Forestry University,Nanjing,Jiangsu 210037;2.Nanjing Rare Bio-tech Development Limited Company,Nanjing,Jiangsu 210037) |
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| Abstract: | Taking Lycoris chinensis which was regenerated from axillary bud in vitro culture seedling as test material,genetic stability of micropropagated plantlets that had been cultured for 27 months was assessed by two different PCR-based markers,ISSRs(Inter-simple sequence repeats)and SRAPs(Sequence-related amplified polymorphisms)markers.The 14 ISSR primers yielded a total of 191 bands(1 novel band)in L.chinensis.SRAP markers were more discriminating than ISSR markers,whereas 15 SRAP primers gave a total of 219 bands(3 polymorphic bands)in micropropagated plants of L.chinensis.The extremely low mutation rate(0.97%)suggested that the established system was appropriate for clonal propagation and preservation of germplasm of Lycoris. |
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| Keywords: | Lycoris chinensis axillary bud in vitro culture system ISSR SRAP somaclone variation |
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