| NH4NO3及碳源对红掌愈伤组织诱导影响的研究 |
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| 引用本文： | 刘炤,刘国锋,张翼. NH4NO3及碳源对红掌愈伤组织诱导影响的研究[J]. 北方园艺, 2011, 0(24): 152-154 |
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| 作者姓名： | 刘炤 刘国锋 张翼 |
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| 作者单位： | 上海植物园上海城市植物资源开发应用工程技术研究中心,上海,200231;华中农业大学,湖北武汉,430070;河南四季春园林艺术工程有限公司,河南郑州,450002 |
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| 摘 要： | 以红掌的根段和叶片为外植体,探讨了不同NH3 NO4浓度和不同碳源组合对外植体愈伤组织诱导率的影响.结果表明:红掌再生对NH4 NO3的浓度非常敏感,低浓度的NH4 NO3有利于诱导愈伤组织,浓度较高时抑制愈伤的诱导；而不同的碳源也会影响到外植体愈伤组织的诱导,蔗糖与葡萄糖相比更适合于红掌愈伤组织的诱导.其中NH4 NO3 0 mg/L、蔗糖3％组合中根和叶片的愈伤组织诱导率最高,分别是92.6％和81.5％.
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| 关 键 词： | 红掌 组织培养 愈伤组织 |
Effect of NH4NO3 and Carbon Sources on the Callus Induction of Anthurium andreanum |
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| LIU Zhao , LIU Guo-feng , ZHANG Yi. Effect of NH4NO3 and Carbon Sources on the Callus Induction of Anthurium andreanum[J]. Northern Horticulture, 2011, 0(24): 152-154 |
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| Authors: | LIU Zhao LIU Guo-feng ZHANG Yi |
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| Affiliation: | LIU Zhao1,LIU Guo-feng2,ZHANG Yi3(1.Shanghai Botanical Garden,Shanghai Engineering Research Center of Sustainable Plant Innovation,Shanghai 200231;2.Huazhong Agricultural University,Wuhan,Hubei 430070;3.Henan Four Season Spring Garden Art Engineering Company Limited,Zhengzhou,Henan 450002) |
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| Abstract: | Anthurium root and leaf was chosen as explants,the effect of different NH4NO3 concentrations and different combinations of carbon sources on the callus induction were studied.The results showed that A.andreanum was sensitive to NH4NO3,a benifical effect of low ammonium/nitrate ratio on growth and shoot production.The carbon source also had impact on callus induction.Sucrose was suitable for callus induction of A.andreanum.The best option was NH4NO3 0 mg/L and sucrose 3%,the calli induction rates of roots and leaves were 92.6% and 81.5%. |
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| Keywords: | Anthurium andraeanum tissue culture callus |
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