不同酿酒葡萄品种白藜芦醇合酶基因的克隆和序列分析

以红葡萄品种“赤霞珠”、“品丽珠”、“黑比诺”和白葡萄品种“雷司令”为试材,采用改良CTAB法提取葡萄叶片组织中的DNA,并扩增得到4个酿酒葡萄品种的白藜芦醇合酶基因部分片段.结果表明:红葡萄品种“赤霞珠”、“品丽珠”、“黑比诺”的扩增产物均为1 419 bp,白葡萄品种“雷司令”扩增产物为1 417 bp.通过序列多重比较分析,所得到的4个白藜芦醇基因片段与Genbank中河岸葡萄(AF128861)的该基因序列同源性较高(90％以上),外显子区同源性高于内含子；红色葡萄姊妹品种之间、红色葡萄不同品种之间、红色葡萄与白色葡萄品种之间的序列同源性依次递减.

Cloning and Sequence Analysis of Resveratrol Synthase Gene from Different Wine Grape Varieties

Red grape varieties ’Cabernet Sauvignon’,’Cabernet Franc’,’Pinot Noir’ and white grape variety ’Riesling’ were used as test material,the DNA from leaf tissue with modified CTAB method were extracted,and get the resveratrol synthase part gene segments of four different wine grape varieties with PCR method.The results showed that amplification product of ’Cabernet Sauvignon’,’Cabernet Franc’,’Pinot Noir’ were all 1 419 bp,yet it was 1 417 bp as for the white grape variety ’Riesling’.The gene sequences homology was quite high(above 90%)between the obtained four gene segments and the same gene of vitis riparia(No.in Genbank:AF128861)according to the sequence multiple comparison analysis.Homology of the exons was higher than introns;when it came to red grape varieties of the same strain,red grape varieties of different strains,red grape varieties and white grape varieties,sequences homology declines successively.