玉米类LFY基因的克隆及其在不同光周期条件下的表达

采用每天9 h和15 h两种光照条件对热带玉米自交系CML288和温带玉米自交系黄早四进行处理。以玉米茎尖为材料，采用RT-PCR方法，分离到1个1 265 bp的全长cDNA克隆，序列比较表明其为拟南芥LFY基因的同源基因，命名为类LFY（基因登录号为DQ343237）。类LFY与同源基因zfl2、RFL、LFY和FLO的同源性依次为93%、84%、57%和57%。它的DNA序列和推论的蛋白质序列与zfl1同源性最高，其编码的蛋白质与zfl1相比在第28、29和160位分别少1个丙氨酸、脯氨酸和苏氨酸，在188位置多1个甲硫氨酸。该基因在2个供试自交系茎尖中长日照条件下不同生长时期持续表达，短日照条件下生长早期和后期不表达，其功能有待进一步研究。

Cloning and Expression Analysis of Maize LFY-like Gene

Two maize(Zea mays L.) inbred lines,CML288 from tropical zone(CIMMYT) and Huangzao 4 from temperate zone(China) were employed in the study.Plants were grown in two different controlled photoperiod conditions with diurnal sunlight of 9 h and 15 h,respectively.A 1 265 bp cDNA(LFY-like) clone was isolated from the stem top of maize by RT-PCR.The LFY-like gene shared a homology of 93% with zfl2,84% with RFL,57% with LFY and FLO.Compared to zfl1,the deduced protein lacks an alanine,proline,theonine in the position of 28th,29th and 160th respectively and has an additional methionine in the position of 188th.Expression analysis showed the gene expressed all time under long-day condition,but not expressed at early or late stage of maize under short-day condition.The function of the gene needs to be studied further.