螺虫乙酯对雌性斑马鱼的毒性及氧化应激效应

为评价新型季酮酸衍生物类杀虫剂螺虫乙酯(spirotetramat)对水生生物的毒性,以雌性斑马鱼为受试对象,研究了螺虫乙酯对其的急性毒性及氧化应激效应。急性毒性试验测得螺虫乙酯对雌性斑马鱼的96 h-LC50值为7.21 mg/L,属中等毒性。经不同浓度螺虫乙酯(0、36、360和1 800μg/L)处理28 d后,通过检测铜/锌超氧化物歧化酶(Cu/Zn-SOD)、锰超氧化物歧化酶(Mn-SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)等抗氧化酶的活性,Cat、Gpx、Mn-Sod、Cu/Zn-Sod等基因的相对表达量,以及丙二醛(MDA)含量等指标的变化水平,研究了螺虫乙酯对斑马鱼肌肉组织的氧化应激效应。结果表明,螺虫乙酯可引起斑马鱼肌肉组织中CAT、GSH-Px活性和MDA含量,以及Cat、Mn-Sod、Gpx的相对表达量显著升高,但未发现螺虫乙酯对Cu/Zn-SOD活性及Cu/Zn-Sod的相对表达量有明显影响。研究表明,螺虫乙酯能够诱导雌性斑马鱼肌肉组织的氧化应激效应,并可能引起机体的氧化损伤。

Toxicity of spirotetramat and oxidative stress response in female zebrafish(Danio rerio)

In order to evaluate the aquatic toxicity of spirotetramat, a new tetramic acid derivative, the acute toxicity and oxidative stress of spirotetramat in female zebrafish(Danio rerio) was studied. The result of acute toxicity test showed that the 96 h-LC50 value of spirotetramat was 7.21 mg/L, suggesting spirotetramat has moderate toxicity to female zebrafish. Oxidative stress was processed by assaying antioxidant enzyme activities(CAT, Cu/Zn-SOD, Mn-SOD and GSH-Px), relative mRNA expression levels of genes(Cat, Cu/Zn-Sod, Mn-Sod, Gpx) and MDA content in zebrafish muscle after exposing to various mass concentrations(0, 36, 360 and 1 800 μg/L) of spirotetramat for 28 d. The results indicated that spirotetramat could induce significant increase of the CAT and GSH-Px activities, MDA content, and mRNA expression levels of Cat, Mn-Sod and Gpx in zebrafish muscle; while the Cu/Zn-Sod mRNA expression level and Cu/Zn-SOD activity was not changed significantly. The results suggested that oxidative stress was induced in zebrafish muscle by spirotetramat, which may cause oxidative damage in the fish.