| 三叶半夏组织培养研究 |
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| 引用本文： | 程霞英,陶晓晔. 三叶半夏组织培养研究[J]. 安徽农业科学, 2010, 38(3): 1643-1645. DOI: 10.3969/j.issn.0517-6611.2010.03.019 |
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| 作者姓名： | 程霞英 陶晓晔 |
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| 作者单位： | 廊坊师范学院生命科学学院,河北廊坊,065000;廊坊师范学院生命科学学院,河北廊坊,065000 |
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| 基金项目： | 河北省教育厅项目(Z2008457);;廊坊师范学院2009年度院级项目(LSZY200902) |
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| 摘 要： | [目的]为三叶半夏组培扩繁寻求最佳培养基配比。[方法]研究正交试验下不同激素浓度和配比对三叶半夏不同外植体诱导分化的影响。[结果]MS4-1．5mg／L6-BA＋0．1mg／LIAA＋0．3mg／LNAA为叶片诱导丛生芽的最佳激素浓度配比，MS＋1．0mg／L6-BA＋0．2mg／LIAA＋0．3mg／LNAA为叶柄诱导丛生芽的最佳激素浓度配比。生根培养基以1／2MS＋0．3mg／LIBA效果较好。[结论]建立了三叶半夏的组培快繁体系。
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| 关 键 词： | 三叶半夏 组织培养 正交试验 |
Study on Tissue Culture of Pinellia ternata |
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| CHENG Xia-ying et al. Study on Tissue Culture of Pinellia ternata[J]. Journal of Anhui Agricultural Sciences, 2010, 38(3): 1643-1645. DOI: 10.3969/j.issn.0517-6611.2010.03.019 |
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| Authors: | CHENG Xia-ying et al |
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| Affiliation: | School of Life Science;Langfang Teachers College;Langfang;Hebei 065000 |
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| Abstract: | [Objective] The aim of the study was to seek the best hormone concentration ratio about inducing clustered shoots of P.ternata.[Method] The inducing effect of different hormone levels on P.ternata with orthogonal test was investigated.[Result]The best hormone concentration ratio of inducing clustered shoots of P.ternata leaf was MS+1.5 mg/L 6-BA+0.1 mg/L IAA+0.3 mg/L NAA,and the one of P.ternata petiole was MS+1.0 mg/L 6-BA+0.2 mg/L IAA+0.3 mg/L NAA.Rooting medium was 1/2 MS+0.3 mg/L IBA.[Conclusion]Tissue ... |
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| Keywords: | Pinellia ternate Tissue culture Orthogonal test |
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